FSCN1与Vimentin在结肠癌中的表达与意义

Expression and significance of FSCN1 and Vimentin in colon cancer

目的探讨FSCN1与Vimentin在人结肠癌中的表达与意义。方法采用免疫组化方法检测人结肠癌及癌旁组织中FSCN1与Vimentin的表达情况,并分析二者在结肠癌中的表达情况与临床病理参数的关系及二者表达的相关性。进一步检测人结肠癌SW-480细胞中FSCN1与Vimentin的调控关系,并研究FSCN1与人结肠癌细胞侵袭转移的关系。结果FSCN1、Vimentin在结肠癌组织中的表达均显著高于癌旁组织(P<0.001)。结肠癌中FSCN1与Vimentin表达呈显著正相关,FSCN1、Vimentin在结肠癌组织中的表达均与TNM分期、淋巴结转移、分化程度均呈正相关(P<0.001)。敲低SW-480细胞中的FSCN1后,VimentinmRNA与蛋白表达水平明显降低(P<0.05)。FSCN1干扰后,SW-480细胞的侵袭、迁移能力明显降低(P<0.05)。结论FSCN1、Vimentin在结肠癌组织中呈高表达,并均与结肠癌转移有关。FSCN1对Vimentin具有正向调控作用,FSCN1可能参与结肠癌细胞EMT过程进而调节结肠癌的转移。

Objective To investigate the expression and value of FSCN1 and Vimentin in human colon cancer.Methods The expression of FSCN1 and Vimentin were detected by immunohistochemical staining in colon cancer and adjacent tissues,and the expression of FSCN1 and Vimen-tin and clinicopathological parameters was analyzed.Furthermore,the regulation of FSCN1 and Vimentin in human colon cancer cell line SW-480 was detected,and the relationship between FSCN1 and invasion and migration of colon cancer cells was researched by cell experiments.Results The expression of FSCN1 and Vimentin in colon cancer tissues were significantly higher than those in adjacent tissues(P<0.001).The expression of FSCN1 and Vimentin in colon cancer was significantly positively correlated,and the expression of FSCN1 and Vimentin in colon cancer tissue was positively correlated with TNM stage,lymph node metastasis and differentiation degree(P<0.001).Knockdown of FSCN1 in SW-480 cells significantly reduced the expression of vimentin mRNA and protein(P<0.05).After the interference of FSCN1,the invasion and migration ability of SW 480 cells decreased significantly(P<0.05).Conclusion FSCN1 and Vimentin are highly expressed in colon cancer,and they are related to the metastasis of colon cancer.FSCN1 has a positive regulatory effect on Vimentin.FSCN1 may be involved in the EMT process of colon cancer cells and then regulate the metastasis of colon cancer.