T2DM心肌纤维化与miRNAs靶点endoglin关联性研究

Relationship between Myocardial Fibrosis and MiRNAs Target Endoglin in T2DM

目的:探究miRNA在T2DM、CVD发生发展中的关联性。方法:通过建立T2DMHe染色观察小鼠心脏的形态学和病理变化、Masson染色观察胶原纤维的表达、QRT-PCR检测mirna-138-5p与各型胶原及胶蛋白的表达、免疫印迹法检测Ⅰ、Ⅲ型胶原的表达。结果:HE及Masson结果表明,相较于对照组,DM组的心肌组织呈紊乱状,明显有胶原的沉积;另外生化免疫结果相比于对照组,除了mirna-138-5p的miRNA出现下调,DM组各型胶原及胶蛋白呈现上调表达,结果有统计学差异。结论:miRNA-385p与Edoglin的调控可能存在一定关联性,结果对临床实践有一定参考意义,推测可以影响mCFs的活性进而调节T2DM心肌纤维化过程。

Objective:To explore the relevance of miRNA in the development of T2DM and CVD.Methods:T2DMHe staining was established to observe the morphological and pathological changes of mouse heart;Masson staining was used to observe the expression of collagen fibers;QRT-PCR was used to detect the expression of mirna-138-5p and various types of collagen and collagen protein;Immunoblotting was used to detect the expression of type Ⅰ and type Ⅲ collagen.Results:The results of HE and Masson showed that compared with the control group,the myocardial tissue of DM group was disordered and collagen deposition was obvious;In addition,the biochemical immune results compared with the control group,except for the miRNA of mirna-138-5p was down-regulated,the expression of collagen and collagen protein in the DM group was up-regulated,and the results were statistically different.Conclusion:Mirna-385p may be related to the regulation of Edoglin.The results have certain reference significance for clinical practice.It is speculated that mirna-385p can affect the activity of mCFs and regulate the process of myocardial fibrosis in T2DM.