基于转录组测序技术的儿茶酚胁迫下酿酒酵母响应机制

Mechanism of Saccharomyces cerevisiae response to catechol stress at transcriptome level

酿酒酵母被认为是发酵纤维素产生乙醇的主要微生物。儿茶酚是木质纤维素水解液中一种主要的酚类抑制物,对酵母细胞的生长代谢具有一定的毒害作用。采用RNA-Seq技术在转录组水平探讨了酿酒酵母在质量分数为1.2 g/L儿茶酚下的响应机制,共筛选出223个差异表达基因(differentially expressed genes,DEGs),其中包括172个上调基因和51个下调基因。结果表明,在儿茶酚胁迫下,酿酒酵母发生了氧化应激,同时通过上调硫代谢、碳代谢以及ABC转运蛋白等通路相关基因来提高酿酒酵母儿茶酚耐受性。研究初步揭示了儿茶酚胁迫下酿酒酵母响应机制,为进一步研究提高酿酒酵母酚类物质耐受性的方法提供了理论依据。

Saccharomyces cerevisiae has developed for ethanol fermentation from lignocellulose.Catechol is a major phenolic inhibitor in lignocellulose acidic hydrolysate,which has toxic on the cells.The RNA-Seq was used to explore the mechanism of S.cerevisiae response to 1.2 g/L catechol at transcriptomic level.A total of 223 genes were identified,172 were up-regulated and 51 were down-regulated.The results indicated that oxidative stress occurred in S.cerevisiae under the catechol stress.Simultaneously,the catechol tolerance of S.cerevisiae could be improved by the up-regulated genes of sulfur metabolism,carbon metabolism and ABC transporter.The results can provide a theoretical basis for further research to improve the tolerance of phenolic substances in S.cerevisiae.