基于CaCO_(3)模板制备大豆蛋白微球及槲皮素的体外释放研究

Preparation of soy protein microspheres based on CaCO_(3) template and in vitro release of quercetin

该研究首次将大豆分离蛋白(soybean protein isolate,SPI)包埋到CaCO_(3)模板上,制备粒径均一、具有递送潜力的蛋白微球。通过控制蛋白的加入量,研究蛋白微球的粒径、蛋白的包埋特性以及蛋白微球的热稳定性能。结果表明,SPI成功地包埋到CaCO_(3)的孔隙以及表面,蛋白质的加入会改善CaCO_(3)的形貌,使其表面更加光滑,分散程度更好,并且可成功地脱去CaCO_(3)模板制得蛋白微球。此外,该研究通过对荧光数据分析发现,制备的蛋白微球具有一定的热稳定性,在80℃下20 min没有发生变性。随后将槲皮素作为被包埋物质应用到蛋白微球内,模拟体外释放特性的研究表明,蛋白微球对槲皮素的延缓释放具有一定的作用。该研究可为提高槲皮素的生物利用度,减少其在体内的损耗提供一种新型的解决方法。

In this paper,the soy protein isolate(SPI)microspheres with uniform particle size and transport potential were prepared by CaCO_(3)templates.The particle size of the protein microspheres,the embedding characteristics of the protein and the thermal stability of the protein microspheres were studied by controlling the amount of SPI.The results showed that SPI was successfully encapsulated in the pores and on the surface of CaCO_(3)templates.Some irregular attachments were formed on the surface of CaCO_(3)without added protein,the surface of CaCO_(3)smoother and the particles more dispersed by adding protein.In addition,the CaCO_(3)templates were successfully removed to prepare protein microspheres.The highest encapsulation rate reached 90.1%when the amount of SPI added up to 10 mg,and the loading rate can reach 48.9μg/mg when the amount of SPI added was 30 mg.This study also found that the prepared protein microspheres have higher thermal stability than SPI,there was no denaturation occurs at 80℃for 20 min.Moreover,quercetin was used as an embedding substance in the preparation of protein microspheres.Through the exploration and simulation of the release characteristics of protein microspheres in vitro,it is shown that the protein microspheres play a certain role in the delayed release of quercetin.This study could provide a novel solution for improving the bioavailability of quercetin and reducing its loss in vivo.