森林草莓糖基转移酶基因家族生物信息学及其表达分析

Analysis of bioinformatics and gene expression of glycosyltransferase gene family in Fragaria vesca

【目的】对森林草莓糖基转移酶基因(FvUGT)家族进行生物信息学分析及基因表达分析,为深入研究森林草莓UGT基因功能和探索UGT调控草莓果实发育及花色苷及品质形成提供理论依据。【方法】基于Phytozome数据库鉴定得出138个FvUGT基因家族基因,运用生物信息学方法分析其蛋白理化性质、基因结构、保守结构域和进化关系,并采用实时荧光定量PCR对UFGT候选基因在森林草莓(红果和白果2个类型)各组织(根、叶柄、叶和果实)中的表达量进行分析。【结果】FvUGT基因家族可分为12个类群(A、C、D、E、F、G、H、I、J、K、L和N),每个类群分别包含16、2、23、32、8、1、5、5、7、3、21和4个成员;染色体定位发现FvUGT基因家族成员在除2号染色体之外的其他染色体上均有分布,且分布不均;FvUGT基因内含子长度、外显子位置和数目在不同成员间均存在差异,FvUGT基因家族在进化过程中产生较强的分化。实时荧光定量PCR检测结果表明,FvUFGT70基因在森林草莓白果和红果的叶和叶柄中有较高表达;FvUFGT94基因在各组织中均有表达;FvUFGT67和FvUFGT68基因在根中有较高表达;而FvUFGT95和FvUFGT96基因在果实中有表达,且显著高于其他组织(P<0.05,下同)。同时,在果实的小绿期、转色期和成熟期的表达表现为,成熟期FvUFGT33和FvUFGT95这2个基因在森林草莓红果类型的表达量显著高于白果类型。【结论】7个FvUFGT基因表现出明显的组织差异性,其中FvUFGT33和FvUFGT95基因在果实中有较高表达量,且在森林草莓红果类型表达量显著高于白果类型,故推测其在花色苷合成中发挥作用。

【Objective】In this study,bioinformatics analysis and gene expression analysis of Fragaria vesca glycosyltransferase gene(FvUGT)family were carried out to provide theoretical basis for the FvUGT gene function of F. vesca and the exploration of UGT regulation of fruit development and anthocyanin and quality formation of F. vesca.【Method】Based on the Phytozome database,138 FvUGT family genes were identified,bioinformatics methods were used to analyze the protein properties,gene structure,conserved domains and evolutionary relationships of these FvUGT genes,and using real-time fluorescence quantitative PCR method to analyze the expression profiles of UFGT candidate genes in various tissues(root,petiole,leaf and fruit)of F. vesca(red fruit and white fruit).【Result】The FvUGT gene family could be divided into 12 subfamilies(A,C,D,E,F,G,H,I,J,K,L and N),each of which contained 16,2,23,32,8,1,5,5,7,3,21 and 4 members. Chromosomal localization analysis revealed that the coding genes of 138 FvUGT genefamily members were distributed on all chromosomes except chromosome 2,and the distribution was uneven. The FvUGT gene intron length,exon position and number were different among different members,and the FvUGT family had a strong differentiation in the process of evolution. qRT-PCR found that the FvUFGT70 gene was highly expressed in leaves and petioles. The FvUFGT94 gene was expressed in various tissues;FvUFGT67 and FvUFGT68 genes had higher expression in roots;FvUFGT95 and FvUFGT96 genes had expression in fruits,which was significantly higher than that of other tissues(P<0.05,the same below). At the fruit’s small green stage,turning stage and ripe stage,and the expression levels of the two genes FvUFGT33 and FvUFGT95 in F. vesca red fruit were significantly higher than white fruit during the ripe stage.【Conclusion】The 7 FvUFGT genes show obvious tissue differences,and FvUFGT33 and FvUFGT95 have high expression in fruit,and the expression in red fruit type is significantly higher than white fruit type. It is speculated that Fv UFGT33 and FvUFGT95 play a role in the synthesis of anthocyanins.