红景天苷对高原冻伤大鼠血管内皮细胞释放舒缩因子的影响研究

Effect of Salidroside on the Release of Vasomotor Factor from Vascular Endothelial Cells of Rats with Frostbite at High Altitude

目的:研究红景天苷对高原冻伤大鼠血管内皮细胞释放舒缩因子的影响。方法:随机选取健康雄性SD大鼠90只,按照随机数字表法分为3组,模拟冻伤组30只,冻伤组30只,红景天苷组30只。红景天苷组定期给予腹腔注射红景天苷20mg/kg,模拟冻伤组和冻伤组定期给予等体积生理盐水注射,从冻伤当日开始,各组均每日注射1次,直到取材截止。冻伤后第1天、第3天、第7天观察各组创面情况,并进行腹主动脉采血,测定血浆中一氧化氮(NO)、血浆内皮素(ET)、血栓素B2(TXB2)、6-酮-前列腺素F1α(6-K-PGF1α)水平。结果:伤后第7天冻区创面情况观察,冻伤组足部组织肿胀明显,各趾远端出现干瘪,近端和远端呈黑色干性坏死,部分坏死组织脱落,无渗出,红景天苷组冻区足部组织和各趾近端肿胀明显,仅远端部分呈黑色干性坏死,无坏死组织脱落,渗出量少。与模拟冻伤组比较,冻伤组NO水平呈先降低后升高再降低的变化,且在伤后第3天最高,第7天时各组比较差异无统计学意义(P>0.05);与冻伤组比较,红景天苷组NO水平在第1天、第3天显著升高,差异具有统计学意义(P<0.05)。与模拟冻伤组比较,冻伤组ET水平都先降低后升高的变化,差异具有统计学意义(P<0.05);与冻伤组比较,红景天苷组ET水平在各时间点均较冻伤组低,差异具有统计学意义(P<0.05)。与冻伤组比较,红景天苷组NO/ET在第1天、第3天差异具有统计学意义(P<0.05)。与模拟冻伤组比较,冻伤组6-K-PGF1α水平呈先升高后降低再升高的变化,差异均有统计学意义(P<0.05);与冻伤组比较,红景天苷组6-K-PGF1α水平在各时间点显著升高,差异具有统计学意义(P<0.05)。与模拟冻伤组比较,冻伤组TXB2水平呈先升高后降低的变化,差异具有统计学意义(P<0.05);与冻伤组比较,红景天苷组TXB2水平在各时相点均低于冻伤组,差异具有统计学意义(P<0.05)。与冻伤组比较,红景天苷组6-K-PGF1α/TXB2的比值在各时间点均高于冻伤组,差异具有统计学意义(P<0.05)。结论:红景天苷对冻区组织具有保护作用,与调节高原地区冻伤大鼠血液中NO/ET以及6-K-PGF1α/TXB2之间的平衡有关,为红景天苷应用于冻伤治疗提供了实验数据。

Objective To study the effect of salidroside on the release of cytokines by vascular endothelial cells in rats with frostbite at high altitude.Methods Randomly selected 90 healthy male SD rats,divided into 3 groups according to the random numerical esqueer,30 rats in the simulated frostbite group,30 rats in the frostbite group,30 rats in the salidroside group.The salidroside group regularly gave abdominal injection of salidroside 20mg/kg,the simulated frostbite group and the frostbite group regularly gave the same volume of physiological saline injection,from the date of frostbite,each group was injected once a day until the end of the material.On the 1st,3rd and 7th days after frostbite,the groups were observed and blood was collected from the abdominal aorta to determine the levels of NO,ET,TXB2,6-K-PGF1αin the plasma.Results After the injury 7d frozen area of the wound surface observation,the frostbite group foot tissue swelling was obvious,the far end of each toe appears dry,near and far end of the black dry necrotasia,part of the necrotic tissue off,no seepage,the salidroside group frozen area foot tissue and the near end of the toe swelling obvious,only the far end of the black dry necrotasia,non-necrotic tissue shedding,oozing less.Compared with the simulated frostbite group,the NO level in the frostbite group showed a change of first decreasing,then increasing and then decreasing,and it was the highest at 3d after injury,and there was no statistically significant difference between each group at 7d(P>0.05).Compared with the frostbite group,NO levels in the salidroside group were significantly increased on 1d and 3d,the differences were statistically significant(P<0.05).Compared with the simulated frostbite group,ET levels in the frostbite group decreased first and then increased(P<0.05).Compared with the frostbite group,ET level in the salidroside group was lower at each phase point than that in the frostbite group(P<0.05).Compared with the frostbite group,the differences of NO/ET in the salidroside group on 1d and 3d were statistically significant(P<0.05).Compared with the simulated frostbite group,the 6-K-PGF1αlevel in the frostbite group increased first,then decreased and then increased(P<0.05).Compared with the frostbite group,the level of 6-K-PGF1αin the salidroside group was significantly increased at each phase point(P<0.05).Compared with the simulated frostbite group,TXB2 level in the frostbite group increased first and then decreased(P<0.05).Compared with the frostbite group,TXB2 level of the salidroside group was lower than that of the frostbite group at all time points(P<0.05).Compared with the frostbite group,the ratio of 6-K-PGF1α/TXB2 in the salidroside group was higher than that in the frostbite group at each phase point(P<0.05).Conclusion Salidroside has a protective effect on frozen tissue,and is related to regulating the balance between NO/ET and 6-K-PGF1α/TXB2 in the blood of frostbite rats in the plateau area,providing experimental data for the application of salidroside to treat frostbite.