LC-MS同时检测白酒中四种人工甜味剂

Simultaneous Detection of Four Sweeteners in Baijiu by LC-MS

实验建立了一种LC-MS同时检测白酒中四种人工甜味剂的方法,白酒样品经蒸发去除乙醇,用超纯水定容,经0.22μm有机滤膜过滤,进质谱检测。采用Poroshell 120 EC-C18柱,以含0.1%甲酸水-甲醇为流动相进行梯度洗脱,在ESI负离子模式下,采用MRM模式进行检测分析。4种甜味剂均有较好的线性关系,线性相关系数R2均大于0.999。检出限在0.2~2μg/L。加标回收率为87.0%~100.9%,该方法能够对白酒中四种人工甜味剂进行准确检测。

In this paper,a method for simultaneous detection of four sweeteners in Baijiu by LC-MS,baijiu samples heated to remove the alcohol,made up to constant volume with ultrapure water,then filtered by 0.22μm filter,and finally analyzed by LC-MS.The samples were gradient-eluted with methanol and 0.1%formic acid as the mobile phase on Poroshell 120 EC-C18 column by using negative ion mode.All the four sweeteners had a good linear relationship and the linear correlation coefficient R2was above 0.999.The detection limit was between 0.2μg/L to 2μg/L,The spiked recoveries were 87.0%to 100.9%.This method can accurately detect four artificial sweeteners in baijiu.