角蛋白/海藻酸钠/聚丙烯酰胺水凝胶皮肤敷料的制备及创口修复研究

Preparation and Wound Repair of Keratin/Sodium Alginate/Polyacrylamide Hydrogel Skin Dressing

为了获得更为理想的皮肤创口修复敷料,在海藻酸钠(SA)和聚丙烯酰胺(PAM)水凝胶的基础上复合人发角蛋白(KTN),制得KTN/SA/PAM水凝胶皮肤敷料。用电子万能测试机、扫描电子显微镜等对其进行表征,结果显示,KTN/SA/PAM水凝胶皮肤敷料拉伸强度为42.41 k Pa,弹性模量11.19 kPa,接近人体皮肤组织;与带血猪皮的黏附性可高达为5.1 kPa,2 h吸水率为144.3%,较好地满足了皮肤创口修复敷料的基本要求。进一步的大鼠皮肤创面修复实验显示,KTN/SA/PAM水凝胶皮肤敷料相对于市售创口贴和SA/PAM水凝胶具有更好的修复能力,创口皮肤组织切片组织学分析表明,经KTN/SA/PAM皮肤敷料处理后的创口处未成熟组织区域最小,胶原纤维排列最为整齐,14 d后,伤口愈合程度与正常皮肤几乎接近。预示KTN/SA/PAM皮肤敷料可能是一种较有前景的皮肤伤口修复敷料。

In order to obtain a more ideal skin wound repair dressing,human hair keratin(KTN)is compounded on the basis of sodium alginate(SA)and polyacrylamide(PAM)hydrogel to prepare KTN/SA/PAM hydrogel glue skin dressing.It was characterized by an electronic universal testing machine,scanning electron microscope,etc.The results showed that the tensile strength of the KTN/SA/PAM hydrogel skin dressing was 42.41 kPa and the elastic modulus was 11.19 kPa,which were close to human skin tissue;The adhesion of the skin can be as high as 5.1 k Pa,and the water absorption rate in 2 hours is 144.3%,which satisfy the basic requirements of skin wound repair dressing.Further rat skin wound repair experiments show that KTN/SA/PAM hydrogel skin dressings have better repairing ability than commercially available wound tapes and SA/PAM hydrogels.Histological analysis of wound skin tissue sections shows that after KTN/SA/PAM skin dressing treatment,the immature tissue areas of the wound were the smallest and the collagen fibers were arranged most neatly.After 14 days,the degree of wound healing was almost similar to that of normal skin.The results indicated that KTN/SA/PAM skin dressing may be a more promising skin wound repair dressing.