α-淀粉酶中某些氨基酸含量与其最适pH的关系

Relation between Percentage of Some Amino Acids and Optimal pH of AlphaAmylases

针对生产中大多数α 淀粉酶不能适应高酸性和高温条件,需要改进才能适应工业生产的实际,研究了α 淀粉酶的最适pH与各种氨基酸含量的关系,构建了α 淀粉酶序列库和pH序列库.通过对α 淀粉酶pH序列库统计分析,发现α 淀粉酶的最适pH与某些氨基酸含量有一定的关系,当丙氨酸(A)质量分数大于5.8%并谷氨酸(E)质量分数大于5.0%,苏氨酸(T)质量分数大于6.5%并酪氨酸(Y)质量分数在4.3%～6.3%时,α 淀粉酶的耐酸性能力较好.

Alphaamylase is an enzyme of great significance for industry. As the enzyme used in the process,most alphaamylases are unstable at lower pH and higher temperature. Consequently, there is a need to develop an enzyme that can operate at industrial conditions. For studying the relation between percentage of kinds amino acids and optimal pH, we built alphaamylase's bank and alphaamylase's pHsequence bank. After analysis, we found that there were 2 kinds cases about relation between percent amino acid and optimal pH: as either(1) A(alanine)%>5.0% and E(glutamic acid)%>5.8%, or (2)T(threonine)%>6.5%, and Y(tyrosine)% in arange of 4.3%～6.3%, alphaamylase had the higher antiacid ability.