白藜芦醇联合γ射线调控PI3K/Akt/mTOR信号通路对宫颈癌Hela细胞的影响

Effect of resveratrol combined with radiotherapy on cervical cancer Hela cell growth and migration by regulating PI3K/Akt/mTOR signaling pathway

目的探讨白藜芦醇联合γ射线对宫颈癌Hela细胞生物学行为的影响,并对其可能机制进行初步探究。方法CCK-8法检测不同浓度白藜芦醇溶液±γ射线照射后细胞群体增殖;划痕实验和Transwell小室实验检测细胞迁移和侵袭;流式细胞术和Annexin V-FITC/PI双染检测细胞凋亡;蛋白印迹法检测PI3K、Akt、p-Akt、mTOR、p-mTOR蛋白表达。结果与对照组相比,白藜芦醇组±γ射线照射均能抑制细胞增殖、迁移、侵袭能力,并促进细胞凋亡,且联合的效果更加明显。与对照组相比,白藜芦醇和γ射线均能降低细胞中Bcl-2、PI3K、p-Akt、p-mTOR蛋白表达,使Bax蛋白表达上调,但Akt、mTOR蛋白未发生明显改变。结论白藜芦醇联合γ射线能够明显抑制Hela细胞增殖、迁移、侵袭能力并促进细胞凋亡,其机制可能与PI3K/Akt/mTOR信号通路的抑制作用及下游相关蛋白的表达相关。

Objective To evaluate the effect of resveratrol combined withγ-ray irradiation on the biological behavior of cervical cancer cells,and to explore its possible mechanism.Methods The proliferation of cell populations after different concentrations of resveratrol solution±γ-ray irradiation was detected by CCK-8 assay.Scratch test and Transwell chamber test were used to detect cell migration and invasion.Flow cytometry and Annexin V-FITC/PI double staining were employed to assess cell apoptosis.Western blot was performed to measure the expression levels of PI3K,Akt,p-Akt,mTOR and p-mTOR proteins.Results Compared with the normal control(NC)group,the resveratrol group±γ-ray irradiation could inhibit the proliferation,migration,and invasion and promote cell apoptosis of human cervical cancer Hela cells,and the combined effect was more obvious.Compared with the NC group,resveratrol andγ-ray irradiation could significantly down-regulate the expression levels of Bcl-2,PI3K,p-Akt and p-mTOR proteins,up-regulate the expression level of Bax protein,but did not significantly alter the expression levels of Akt and mTOR proteins in human cervic1255al cancer Hela cells.Conclusions Resveratrol combined withγ-ray irradiation can dramatically inhibit the proliferation,migration,invasion,and promote the apoptosis of cervical cancer Hela cells.The mechanism may be related to the inhibition of the PI3K/Akt/mTOR signaling pathway and down-regulating the expression levels of downstream related proteins.