粗壮女贞苦丁茶提取物对油脂氧化的抑制作用

Inhibition effect of Ligustrum robustum(Rxob.) Blume extract on the oxidation of oil

目的研究以乙醇作为溶剂的粗壮女贞苦丁茶提取物在油脂氧化过程中的抑制作用。方法以花生油为模型,通过测定和比较其在贮藏过程中的过氧化值变化来评价提取物抑制油脂酸败的能力;通过体外化学分析的方法测定提取物的自由基清除能力、脂质过氧化抑制能力和还原能力来探究其抗氧化能力;最后通过测定其总酚和总黄酮含量,并采用高效液相色谱-质谱联用法分析鉴定提取物中的主要活性成分。结果粗壮女贞苦丁茶提取物能有效减缓油脂过氧化值的升高,抑制油脂的氧化酸败;提取物展现出良好的自由基清除活性、脂质过氧化抑制能力和还原能力;提取物中的总酚和总黄酮含量较高,分别达到了126.9mg没食子酸当量/g提取物和135.6 mg槲皮素当量/g提取物;粗壮女贞苷B是提取物中的主要活性成分,经测定其含量为765.83 mg/g提取物,该物质也是提取物能在油脂中发挥抗氧化作用的重要原因。结论粗壮女贞苦丁茶提取物能有效抑制油脂的氧化酸败,在油脂的加工和贮藏领域有潜在的应用价值。

Objective To study the inhibitory effect of the extract of Ligustrum robustum(Rxob.) Blume with ethanol as the solvent in the process of oil oxidation. Methods Taking peanut oil as a model, the ability of extracts to inhibit the rancidity of oils was evaluated by measuring and comparing the changes in peroxide value during storage. Then the free radical scavenging activity, lipid peroxidation inhibition, reducing power of extract were determined by in vitro chemical analysis methods. Finally, the contents of total phenols and total flavonoids were determined, and the main active components were identified by high performance liquid chromatography-mass spectrometry. Results The extract of Ligustrum robustum(Rxob.) Blume could effectively slow down the increase of lipid peroxide value and inhibit the oxidative rancidity of oil during its storage. Moreover, Ligustrum robustum(Rxob.) Blume extract showed the remarkable free radical scavenging activity, lipid peroxidation inhibition ability and reducing power. The content of total phenols and total flavonoids in the extract was relatively high, reaching 126.9 mg gallic acid equivalent/g extract and 135.6 mg quercetin equivalent/g extract, respectively. Ligurobustoside B was the main active ingredient in the extract, and its content was determined to be 765.83 mg/g extract. This substance was also an important reason why the extract can exert an antioxidant effect in oil. Conclusion The extract of Ligustrum robustum(Rxob.) Blume can effectively inhibit the oxidation and rancidity of oil and has potential application value in the field of oil processing and storage.