干露时间对凡纳滨对虾抗氧化功能的影响

Effects of Air Exposure Period on Antioxidant Function of Pacific White Shrimp Litopenaeus vannamei

将体长(8.32±1.43)cm健康有活力的凡纳滨对虾置于室温25℃的封闭实验台上,0、5、15、30 min后分别活体采样,测定肌肉、心脏、胃、肠道、肝胰腺和血淋巴的抗氧化相关指标,研究干露对凡纳滨对虾抗氧化功能的影响。试验结果表明,干露15min组凡纳滨对虾胃、血清、肝胰腺丙二醛含量显著高于对照组(P<0.05);随着暴露时间延长,干露组凡纳滨对虾肝胰腺组织总抗氧化能力呈下降趋势,各组与对照组相比显著降低(P<0.05);肠道、心脏和胃总抗氧化能力水平呈先升后降的趋势;随着暴露时间的延长,凡纳滨对虾肝胰腺和肌肉中总超氧化物歧化酶以及肝胰腺中γ-谷氨酰半胱氨酸合成酶活性呈下降趋势,肌肉与肠道中的γ-谷氨酰半胱氨酸合成酶活性无显著性变化(P>0.05)。短时间(5min)干露对凡纳滨对虾组织抗氧化功能破坏较小,而长时间(30min)干露可明显降低组织的抗氧化能力;干露对凡纳滨对虾对抗氧化功能的影响具有组织特异性。通过检测凡纳滨对虾抗氧化代谢指标,进而探索缺氧不同时间段凡纳滨对虾抗氧化酶活性的变化规律,总结对虾机体抗氧化剂和抗氧化能力的时空图谱,为养殖过程中缺氧现象提供基础资料,为凡纳滨对虾无水运输技术的开发提供参考数据。

The antioxidant related indicators were determined in muscle,heart,and stomach,intestine,hepatopancreas and hemolymph of live healthy and vigorous Pacific white shrimp Litopenaeus vannamei with body length of(8.32±1.43)cm with air exposure on a closed laboratory table for 0,5,15,and 30 min at a room temperature of 25℃to investigate the effects of air exposure on the activities of antioxidant function of Pacific white shrimp.It was found that there was significantly higher malondialdehyde content in Pacific white shrimp in 15 min air exposure than that in the control group(P<0.05),with downward trend of total antioxidant capacity(TAC)in hepatopancreas with the increase in air exposure time,compared with the control group.The TAC levels in the intestine,heart,and stomach were shown to be increase first and then decreased with the increase in air exposure period,and the activity of total superoxide dismutase in hepatopancreas and muscle tissues andγ-glutamylcysteine synthetase activity in hepatopancreas tissues were decreased,without significantly change in activity of muscle and intestinal tissues(P>0.05).It can be concluded that short-term(5 min)air exposure led to has less damage to the antioxidant function of Pacific white shrimp and that prolonged(30 min)air exposure can significantly reduce the antioxidant capacity of the tissue.The spatio-temporal changes in antioxidant capacity provide the basic information on auxiliary reference for the development of the air-exposure transportation technology of Pacific white shrimp.