复合微生物净水剂在仿刺参养殖中的应用

Application of a Compound Microbial Water Purification Agent in Culture of Sea Cucumber Apostichopus japonicus

为探究光合细菌与芽孢杆菌复合微生物制剂的净水能力,以实验室分离保存的1株光合细菌PSB1和1株芽孢杆菌BS1为研究对象,通过测量人工污水中氨氮质量浓度、硫化物质量浓度、化学需氧量的变化确定2株菌混合的最优密度配比为2∶1。在该混合配比下,污水中氨氮的质量浓度由4.68 mg/L降至0.39mg/L,硫化物质量浓度由0.62mg/L降至0.26mg/L,化学需氧量由4.02mg/L降至2.35mg/L。安全性试验表明,复合微生物净水剂砷含量为0.017mg/kg,镉含量为1.634×10^(-4) mg/kg,均低于国家标准中规定的含量,且未检测出黄曲霉素B_(1)、志贺氏菌等成分,符合安全标准。为进一步探究复合微生物净水剂对养殖污水水质的影响,研究在仿刺参养殖试验过程中投放该复合微生物净水剂,测量养殖污水中化学需氧量及氨氮、硫化物质量浓度变化。结果表明,在水体pH7.0~8.5、盐度25~35时,复合微生物净水剂对氨氮、硫化物和化学需氧量均有很好的降解作用。

In order to study the purification capacity of the compound microbial preparations of photosynthetic bacterial Rhodopseudomonas palustris PSB1 and Bacillus BS1 isolated and preserved in the laboratory were taken as the research objects.After measuring the content changes in levels of ammonia nitrogen,and sulfide,and chemical oxygen demand(COD)in artificial sewage,the optimal density ratio of the two strains was 2∶1.Under this ratio,the ammonia nitrogen content in sewage was decreased from 4.68 mg/L to 0.39 mg/L,the sulfide content decreased from 0.62 mg/L to 0.26 mg/L,and the COD decreased from 4.02 mg/L to 2.35 mg/L.The safety test showed that the contents of arsenic and cadmium in the compound microbial water purification agent were 0.017 mg/kg and 1.634×10^(-4) mg/kg,lower than the national standard,and no aflatoxin B_(1),Shigella and other components were detected to meet the safety standard.In order to further explore the influence of composite microbial water purification agent on the quality of aquaculture wastewater,the microbial water purification agent was put into the sea cucumber Apostichopus japonicus culture to measure the concentration changes in COD,ammonia nitrogen and sulfide.It was found that the compound microbial water purifier had a good degradation effect on ammonia nitrogen,sulfide,and COD at pH of 7.0—8.5 and asalinity of 25—35.