固定化脂肪酶催化制备甘油三酯型鱼油

Preparation of triglyceride fish oil catalyzed by immobilized lipase

以固定化脂肪酶作为催化剂,以甘油和鱼油乙酯为原料进行酯交换反应制备甘油三酯型鱼油。通过单因素试验考察了酶种类、真空度、反应温度、加酶量对酯交换反应的影响,并采用正交试验对酯交换反应条件进行了优化。结果表明:固定化脂肪酶催化制备甘油三酯型鱼油的最佳反应条件为Novozym 435脂肪酶作为催化剂、真空度500 Pa、反应温度50℃、加酶量2%、反应时间15 h,在该条件下产物中甘油三酯含量达到78%,经过分子蒸馏后甘油三酯含量达到82.08%;脂肪酶重复使用15次,酯交换反应后产物中甘油三酯含量仍能达到74%。

With immobilized lipase as catalyst,the triglyceride fish oil was prepared from fish oil ethyl esters and glycerol by transesterification.The effects of enzyme species,vacuum degree,reaction temperature,enzyme dosage on transesterification were investigated by single factor experiment,and the transesterification conditions were optimized by orthogonal experiment.The results showed that the optimal conditions of preparing triglyceride fish oil catalyzed by immobilized lipase were obtained as follows:Novozym 435 lipase as catalyst,vacuum degree 500 Pa,reaction temperature 50℃,enzyme dosage 2%,reaction time 15 h.Under these conditions,the triglyceride content reached 78%,and it could reach 82.08%after molecular distillation.The triglyceride content could reach 74%when the immobilized lipase was used for fifteen times.