清消法通过调节TLR-2/NF-κB通路对大鼠痤疮局部炎症反应的改善作用研究

Improving Function of Qing Xiao Method on Local Inflammatory Response of Acne in Rats by Regulating TLR-2/NF-κB pathway

目的:观察清消法对痤疮模型大鼠耳廓炎症反应的影响,探讨清消法治疗痤疮的作用机制。方法:将40只大鼠随机分为空白组、模型组、西药组和中药组各10只,采用大鼠右耳廓注射痤疮丙酸杆菌方法复制痤疮模型。中药组给予枇杷清肺饮加减方,西药组给予异维A酸胶囊灌胃,其他2组给予等剂量生理盐水灌胃,连续给药21 d后观察大鼠耳外观及形态变化,测量大鼠耳廓厚度,计算大鼠耳廓组织肿胀率,ELISA检测大鼠血清中TNF-α、IFN-γ含量,免疫组化和蛋白质印迹(Western blot)检测大鼠病灶组织中的TLR-2、NF-κB P65蛋白表达。结果:与空白组比较,模型组大鼠耳廓厚度及肿胀率显著增高,血清TNF-α升高,IFN-γ降低,大鼠耳廓组织中TLR-2、NF-κB的表达明显增加(均P<0.05);与模型组比较,西药组和中药组大鼠耳廓组织厚度及肿胀率、血清TNF-α、IFN-γ表达水平与大鼠耳廓组织TLR-2、NF-κB的表达水平均有所改善(均P<0.05),且中药组的改善效果稍好于西药组。结论:清消法能有效地改善大鼠痤疮局部炎症反应,其机制可能通过调节TLR-2/NF-ΚB来抑制TNF-α表达、促进IFN-γ表达。

Objective:To observe the effect of Qingxiao method on auricle inflammation in acne model rats,and to explore the mechanism of Qingxiao method in the treatment of acne.Methods:Forty rats were randomly divided into blank group,model group,western medicine group and traditional Chinese medicine group with 10 rats in each group.The acne model was established by injecting Propionibacterium acnes into the right auricle of rats.The traditional Chinese medicine group was given pipa Qingfei Yin addition and subtraction prescription,the western medicine group was given isoviroic acid capsule intragastrically,and the other two groups were given the same dose of normal saline intragastrically.After 21 days of continuous administration,the appearance and morphology of rats were observed,the thickness of auricle was measured,the swelling rate of auricle tissue was calculated.The contents of TNF-αand IFN-γin serum were detected by ELISA,and the expression of TLR-2,NF-κB p65 protein in rat lesions were detected by immunohistochemistry and Western blot.Results:Compared with the blank group,the auricle thickness and swelling rate of the model group were significantly increased,the serum TNF-αwas increased,the IFN-γwas decreased,and the expression of TLR-2,NF-κB in the auricle tissue of the rats was significantly increased(all P<0.05).Compared with the model group,the thickness and swelling rate of auricle tissue,the expression level of TNF-αand IFN-γin serum and the expression level of TLR-2 and NF-κB in auricle tissue of rats in the western medicine group and the traditional Chinese medicine group were improved(P<0.05).The improvement effect of traditional Chinese medicine group was slightly better than that of Western medicine group.Conclusion:Qingxiao method can effectively improve the local infLlammatory response of acne in rats,and its mechanism may be to regulate TLR-2/NF-κB to inhibit the expression of TNF-αand promote the expression of IFN-γ.