儿童社区获得性肺炎TIM-1基因启动子多态性

Genetic polymorphisms of TIM-1 gene promoter in children with community-acquired pneumonia

目的探究T细胞免疫球蛋白黏蛋白-1(TIM-1)基因启动子遗传多态性对本地区儿童社区获得性肺炎(CAP)易感性的影响。方法选择河南医学高等专科学校附属医院急诊及儿科住院病房2019年6月-2020年3月收治的CAP患儿106例作为病例组,选择同期医院进行体检的健康儿童80名作为对照组,通过电子病历调查收集纳入患儿临床资料。采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)检测TIM-1基因rs41297579 G>A位点多态性分布。结果病例组患者TIM-1基因rs41297579 G>A位点AA基因型及A等位基因频率(29.25%、48.58%)高于对照组,GG基因型频率(32.08%)低于对照组(P<0.05);重症CAP患儿AA基因型及A等位基因频率(47.50%、61.25%)高于轻症组,差异均具有统计学意义(P<0.05);TIM-基因rs41297579 G>A位点携带不同基因型CAP患儿发热时间、混合病原感染比较差异具有统计学意义(P<0.05),住院时间、合并呼吸困难比较,无统计学差异;AA组发热时间长于GG组及GA组,混合病原感染比例高于GA组及GG组(P<0.05);TIM-1基因rs41297579 G>A位点携带不同基因型CAP患儿白细胞计数(WBC)、C-反应蛋白(CRP)、降钙素原(PCT)整体水平比较差异具有统计学意义(P<0.05),AA组WBC、CRP、PCT水平高于GG组及GA组(P<0.05)。结论TIM-1基因rs41297579 G>A位点AA基因型及A等位基因显著增加了本地区儿童对CAP的易感性,且与患儿病情存在关联,具有一定的临床价值。

OBJECTIVE To explore the influence of genetic polymorphisms of T cell immunoglobulin mucin-1(TIM-1)gene promoter on the children’s susceptibility to community-acquired pneumonia(CAP)in this region.METHODS A total of 106 children with CAP who were treated in emergency department and hospitalized in pediatrics department of Affiliated Hospital of Henan Medical College from Jun 2019 to Mar 2020 were assigned as the case group,meanwhile,80 healthy children who received physical examination were set as the control group.The clinical data were collected from the children through review of electronic medical records.The polymorphisms at rs41297579 G>A locus of TIM-1 gene were detected by using restriction fragment length polymorphism polymerase chain reaction(PCR-RFLP).RESULTS The frequencies of genotype AA at rs41297579 G>A locus of TIM-1 gene and allele A of the case group were respectively 29.25%and 48.58%,significantly higher than those of the control group;the frequency of genotype GG of the case group was 32.08%,significantly lower than those of the control group(P<0.05).The frequencies of genotype AA and allele A of the children with severe CAP were respectively 47.50%and 61.25%,significantly higher than those of the children with mild CAP(P<0.05).There were significant differences in the time of fever and mixed infections among the CAP children carrying with different genotypes at rs41297579 G>A locus of TIM-1 gene(P<0.05),while there were no significant differences in length of hospital stay and complication with dyspnea.The time of fever of the AA group was longer than that of the GG group and the GA group,the proportion of the patients with mixed infection was significantly higher in the AA group than in the GA group and the GG group(P<0.05).There were significant differences in the overall levels of white blood cell(WBC),C-reactive protein(CRP)and procalcitonin(PCT)among the CAP children carrying with different genotypes at rs41297579 G>A locus of TIM-1 gene(P<0.05);the levels of WBC,CRP and PCT of the AA group were significantly higher than those of the GG group and the GA group(P<0.05).CONCLUSION The genotype AA at rs41297579 G>A locus of TIM-1 gene and allele A may significantly increase the children’s susceptibility to CAP in this region and are associated with the illness condition of the children,and they have certain clinical value.