半枫荷抗炎镇痛活性部位筛选及其成分分析

Screening and Component Analysis of Anti-inflammatory andAnalgesic Active Parts of Banfenghe

目的研究半枫荷的药理活性,筛选其抗炎镇痛作用的活性部位,并对其化学成分进行分析。方法通过二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔通透性增加试验以及醋酸致小鼠扭体、热板致小鼠疼痛试验对半枫荷乙醇提取浸膏、石油醚部位组、乙酸乙酯部位组、正丁醇部位组和水溶部位组的抗炎、镇痛活性进行筛选。采用LC-MS/MS分析鉴定活性部位化学成分。结果不同剂量的半枫荷乙醇提取浸膏和乙酸乙酯部位组可以明显减轻二甲苯所致小鼠耳肿胀,抑制醋酸致小鼠腹腔毛细血管通透性增加;高剂量半枫荷乙醇提取浸膏和乙酸乙酯部位组灌胃给药能明显减少醋酸致小鼠扭体次数,延长热板法致小鼠的痛阈值,但醋酸扭体法的镇痛效果远不如热板法中的镇痛效果,说明其镇痛作用可能主要为中枢镇痛。通过LC-MS/MS快速鉴别分析乙酸乙酯部位化学成分,初步鉴定出44种化合物。结论乙酸乙酯部位是半枫荷的主要活性部位,具有明显的抗炎、镇痛作用;乙酸乙酯部位的化学成分主要包含黄酮类、三萜类、环烯谜萜类、单萜类等物质。本研究为半枫荷药用资源的深入开发利用提供了科学依据。

Objective This paper studies the pharmacological activity of Banfenghe from the perspective of anti-inflammatory and analgesic, screens the active parts of its anti-inflammatory and analgesic effect, and analyzes its chemical components.Methods The anti-inflammatory and analgesic activities of Banfenghe ethanol extract, petroleum ether part group, ethyl acetate part group, n-butanol part group and water-soluble part group were screened by xylene induced mouse ear swelling, acetic acid induced mouse abdominal permeability increase test, acetic acid induced mouse writhing and hot plate induced mouse pain test.LC-MS/MS analysis was used to identify the chemical components of the active parts.Results Different doses of alcohol extract of Banfenghe and ethyl acetate could significantly reduce the ear swelling induced by xylene and inhibit the increase of peritoneal capillary permeability induced by acetic acid;Intragastric administration of high-dose Banfenghe ethanol extract and ethyl acetate can significantly reduce the number of writhing induced by acetic acid and prolong the pain threshold of mice induced by hot plate method, but the analgesic effect of acetic acid writhing method is far less than that of hot plate method, which indicating that its analgesic effect may be mainly central analgesia.44 compounds were identified by LC-MS/MS.Conclusion Ethyl acetate fraction is the main active fraction of Banfenghe, which has obvious anti-inflammatory and analgesic effects;The chemical constituents of ethyl acetate mainly include flavonoids, triterpenes, cycloterpenes, monoterpenes and so on.This study provides a scientific basis for the further development and utilization of medicinal resources of Banfenghe.