网络药理学预测人参抗抑郁作用机制

Prediction of Antidepressant Mechanism of Ginsengby Network Pharmacology

目的研究人参治疗抑郁症的潜在分子机制。方法通过网络药理学的方法,采用TCMSP数据库查询获取人参主要的活性成分,利用Drugbank、GeneCards、NCBI、OMIM等数据库筛选人参活性成分并预测人参抗抑郁的作用靶点。使用String数据库和Cytoscape软件绘制靶点蛋白相互作用网络,使用Cytoscape软件构建活性成分-作用靶点的网络,采用DAVID数据库对靶点进行GO及KEGG通路分析。结果经过筛选得到人参的活性成分17个,主要涉及的作用靶点有43个。网络分析结果表明,人参发挥抗抑郁药效过程中主要涉及代谢过程、细胞过程、对应激的应答等生物过程,通过调节Neuroactive ligand-receptor interaction、Toxoplasmosis、Pathways in cancer、Leishmaniasis、Serotonergic synapse、Osteoclast differentiation、Tuberculosis等信号通路来发挥抗抑郁作用。结论人参中的活性化合物延胡索碱、豆甾醇、人参二醇山奈酚、花生四烯酸等能作用于AKT1、TNF、PPARG、APRB2、IL-1β、PTGS2等靶点调节多条信号通路进行抗炎、调节突触等作用,从而可能发挥对抑郁症的治疗作用。

Objective To study the potential molecular mechanism of Ginseng in the treatment of depression.Methods Through the method of network pharmacology,the main active components of Ginseng were obtained by TCMSP database.The active components of Ginseng were screened by Drugbank,Genecards,NCBI,OMIM and other databases,and the antidepressant targets of Ginseng were predicted.String database and Cytoscape software were used to draw the target protein interaction network,Cytoscape software was used to construct the network of active ingredient action target,and DAVID database was used to analyze the go and KEGG pathways of the target.Results After screening,17 active components of Ginseng were obtained,and 43 targets were mainly involved.The results of network analysis showed that Ginseng mainly involved biological processes such as metabolic process,cellular process and response to stress in the process of exerting antidepressant effect,through regulating neuroactive live receptor interaction,toxoplasmosis,pathways in cancer,leishmaniasis,serotonic synapse,osteolast differentiation tuberculosis and other signaling pathways to play an antidepressant role.Conclusion Fumarine,stigmasterol,Panaxadiol,kaempferol and arachidonic acid can act on AKT1,TNF,PPARG,aprb2 and IL-1β、PTGS2 and other targets regulate multiple signal pathways for anti-inflammatory and synaptic regulation,which may play a role in the treatment of depression.