基于肌肉细胞水凝胶纤维的光固化生物打印系统设计

Design of Photocurable Bio-printing System Based on Muscle Cell Hydrogel Fiber

肌肉纤维是生物致动器的重要动力来源,但目前仍缺少合适的设备制备肌肉细胞纤维。生物打印因其高精度、生物兼容性好的特点被广泛应用于构造生物细胞组织。该文设计开发了一种基于光固化的生物打印系统,打印了载有C2C12肌肉细胞的GelMA水凝胶纤维,分析了不同紫外光强和水凝胶流量下的可打印范围、纤维平均线宽和均匀性。实验结果表明,在光强为20 mW/cm^(2)和流量为120μL/min时,纤维的效果最佳,同时细胞的存活率很高。该研究对生物致动器的研究具有重要意义。

Muscle fibers are an important power source of biological actuators,but there is still a lack of suitable equipment to prepare muscle cell fibers.Bioprinting has been widely used in the construction of biological cells and tissues due to its high precision and good biocompatibility.In this paper,a photocurable bio-printing system was designed and developed.GelMA hydrogel fibers loaded with C2C12 muscle cells were printed.The printable range,fiber average line width and uniformity were analyzed under different ultraviolet light intensity and hydrogel flow.The experimental results showed that the optimum fiber efficiency and cell survival rate were obtained when the light intensity was 20 mW/cm^(2) and the flow rate was 120μL/min.This study is of great significance to the study of biological actuators.