摘要
目的建立可行的院内制剂茵芩合剂的薄层色谱鉴别方法。方法采用微乳液(十二烷基硫酸钠(SDS)-正丁醇-油相-水=2.7∶6.3∶1.0∶30.0,质量比w/w)为基础展开体系,同时对制剂的主要成分茵陈、黄芩和大黄进行薄层鉴别,考察以正庚烷、正已烷和环已烷为油相,以及添加甲酸、丙酮作为改性剂时对分离鉴别效果的影响。结果以正庚烷、正已烷和环已烷为油相配制的微乳液均有较好的分离鉴别效果,但以微乳液(SDS-正丁醇-环已烷-水=2.7∶6.3∶1.0∶30.0(w/w))-甲酸=9∶1为更好。结论采用微乳薄层色谱法可同时鉴别茵芩合剂的多种组分,操作简单,效果可靠。
OBJECTIVE To establish a method for the identification of YinQin mixture by TLC.METHODS The microemulsion(Sodium Lauryl sulfate-n-butanol-oil phase-water=2.7∶6.3∶1.0∶30.0,w/w)was used as the basic developing system,and the main components of the preparation were identified by TLC,the effects of N-heptane,n-hexane and cyclohexane as oil phases and the addition of formic acid and acetone as modifiers on the separation and identification were investigated.RESULTS The microemulsions prepared with N-heptane,n-hexane and cyclohexane as oil had good separation and identification results,but the microemulsions(SDS-n-butanol-cyclohexane-water=2.7∶6.3∶1.0∶30.0(w/w))-formic acid 9∶1 were better.CONCLUSION Microemulsion Thin layer chromatography can be used to identify the multiple components of YinQin mixture.The operation is simple and the effect is reliable.
作者
欧人豪
李萍
曾金
韦祖巧
OU Ren-hao;LI Ping;ZENG Jin;WEI Zu-qiao(Department of Pharmacy,Liuzhou Maternity and Child Healthcare Hospital,Affiliated Maternity Hospital and Affiliated Children′s Hospital of Guangxi University of Science and Technology,Liuzhou 545001,China)
出处
《海峡药学》
2021年第8期46-48,共3页
Strait Pharmaceutical Journal
基金
广西卫计委自筹经费科研课题(合同号:Z 20170522)。
关键词
微乳薄层色谱法
茵芩合剂
鉴别
Microemulsion Thin-layer chromatography
YinQin mixture
Ldentification