结直肠癌组织PEA15、MSI-2的表达与肝转移及预后的关系

Expression of PEA15 and MSI-2 in colorectal cancer tissues and its relationship with liver metastasis and prognosis.

目的分析星形胶质细胞磷酸化蛋白15(PEA15)、MSI-2在结直肠癌组织中的表达及其与结直肠癌肝转移及预后的关系。方法选取2015年1月—2017年12月江苏省泰州市人民医院/南通大学第五附属医院消化内科收治结直肠癌患者180例,应用免疫组织化学染色法检测并比较结直肠癌组织及其癌旁组织中PEA15、MSI-2表达差异。比较结肠癌组织中PEA15、MSI-2在不同临床病理特征中的表达差异。对患者随访3年,分析不同PEA15、MSI-2表达与患者预后的关系。结果结直肠癌组织PEA15、MSI-2阳性表达率显著高于癌旁组织(χ^(2)/P=96.288/0.000、118.063/0.000)。中低分化、原发癌灶浸润深度T3~T4、淋巴结转移患者结直肠癌组织中PEA15、MSI-2阳性表达率显著高于高分化、原发癌灶浸润深度T1~T2、无淋巴结转移患者(PEA15:χ^(2)/P=39.470/0.000、6.668/0.010、19.256/0.000;MSI-2:χ^(2)/P=33.846/0.000、8.638/0.003、27.262/0.000)。肝转移患者PEA15、MSI-2阳性表达率显著高于无肝转移患者(χ^(2)/P=18.299/0.000、16.166/0.000)。结直肠癌患者癌组织中PEA15表达与MSI-2表达呈正相关(r/P=0.575/0.000)。结直肠癌组织PEA15阴性、MSI-2阴性患者生存曲线显著优于PEA15阳性、MSI-2阳性患者(χ^(2)/P=26.436/0.000、22.896/0.000)。结论PEA15、MSI-2高表达与结直肠癌发生发展及肝转移有密切关系,且两者表达呈正相关,PEA15、MSI-2阳性表达患者总体预后较PEA15、MSI-2阴性表达患者差。

Objective To investigate the expression of astrocyte phosphorylated protein 15(PEA15)and MSI-2 in colorectal cancer tissues,and to analyze their relationship with liver metastasis and prognosis of colorectal cancer.Methods 180 patients with colorectal cancer in Digestive System Department of Taizhou People's Hospital,Jiangsu Province(The Fifth Affiliated Hospital of Nantong University)from January 2015 to December 2017 were selected.The expression of PEA15 and MSI-2 in colorectal cancer tissues and adjacent tissues were detected by immunohistochemical staining,and the relationship between the expression of PEA15 and MSI-2 and the clinicopathological characteristics of patients and the correlation of their expression were analyzed.Among them,38 patients with liver metastasis were classified as the liver metastasis group,and 142 patients without liver metastasis were classified as the non-liver metastasis group.The expression of PEA15 and MSI-2 in colorectal cancer tissues of the liver metastasis group and the non-liver metastasis group were analyzed.The patients were followed up for 3 years,the survival status of patients with different expression of PEA15 and MSI-2 were analyzed.Results The positive expression rates of PEA15 and MSI-2 in colorectal cancer tissues of patients with colorectal cancer were significantly higher than those in adjacent tissues(χ^(2)/P=96.288/0.000,118.063/0.000).The positive expression rates of PEA15 and MSI-2 in colon cancer tissues of patients with medium and low differentiation,T3-T4 invasion depth of primary cancer and lymph node metastasis were significantly higher than those of patients with high differentiation,T1-T2 invasion depth of primary cancer and no lymph node metastasis(χ^(2)/P=39.470/0.000,6.668/0.010,19.256/0.000,33.846/0.000,8.638/0.003,27.262/0.000).The positive expression rates of PEA15 and MSI-2 in liver metastasis group were significantly higher than those in non-liver metastasis group(χ^(2)/P=18.299/0.000,16.166/0.000).Pearson correlation analysis showed that the expression of PEA15 in colorectal cancer tissues of patients with colorectal cancer was positively correlated with the expression of MSI-2(r/P=0.575/0.000).The survival curve of patients with colorectal cancer with negative PEA15 and negative MSI-2 were significantly better than those with patients with positive PEA15 and positive MSI-2(χ^(2)/P=26.436/0.000,22.896/0.000).Conclusion The high expression of PEA15 and MSI-2 are closely related to the occurrence and liver metastasis of colorectal cancer.The expression of they are closely related to the differentiation degree,the depth of tumor invasion and lymph node metastasis of colorectal cancer,and the expression of them is positively correlated.The overall prognosis of patients with positive expression of PEA15 and MSI-2 are worse than those with patients with negative expression of PEA15 and MSI-2.