人和猕猴Broca区跨物种解剖连接差异分析

Analysis of Cross-species Anatomical Connection Differences Between Human and Macaque Broca Region

基于比较神经科学的思想,采用脑图谱配准法从人和猕猴的每一个被试大脑中提取了Broca目标脑区和10个感兴趣区域,构建了从被试大脑的Broca脑区到10个感兴趣区域之间的连接性指纹图,从大脑连接结构的角度对人脑和猕猴大脑中的Broca区进行了跨物种连接差异分析。针对样本特征分布不均匀的问题,运用了独立样本t检验方法,从物种总体均值的角度对Broca脑区与某一目标大脑区域之间的连接差异进行了分析。针对样本数量少、对结果刻画能力不足的问题,运用了排列置换测试的统计学分析方法,通过大量计算,对实验结果的可信度进行了分析。此外,还利用人脑和猕猴被试大脑中的同源纤维束为Broca区重新构建了连接性指纹,将生成的指纹图与上述结果进行对比分析。最终结果显示,人脑和猕猴大脑Broca脑区与大多数感兴趣脑区之间的总体均值差异较小,两物种连接性指纹的变化趋势拟合度高,这表明在解剖学上同源的Broca区在连接结构方面也具有较高的跨物种一致性。

On the basis of the idea of comparative neuroscience,the brain atlas registration method was used to extract Broca target brain regions and 10 regions of interest from each subject brain of humans and macaques,and a connectivity fingerprint was constructed from the Broca area of the subject's brain to 10 target brain areas.From the perspective of brain connection structure,cross-species connection difference of Broca regions in human brain and macaque brain was analyzed.Aiming at the problem of uneven distribution of sample characteristics,the independent sample t test method was used to analyze the connection difference between the Broca brain area and a certain target brain area from the perspective of the overall mean value of the species.Aiming at the problem of small sample size and insufficient ability to describe the results,the statistical analysis method of permutation test was used,and the reliability of the experimental results was analyzed through a large number of calculations.In addition,the homologous fiber bundles in the brains of human and rhesus monkeys were used to reconstruct the connectivity fingerprints of the Broca area,and the generated fingerprints were compared and analyzed with the above results.The final results shows that the overall mean difference between the Broca brain regions of human and macaque brains and most brain regions of interest is small,and the change trend of the connectivity fingerprints of the two species has a high degree of fit,which indicates that anatomically homologous Broca region also has high cross-species consistency in connection structure.