金鱼源维氏气单胞菌JY01全基因组测序及生物信息分析

Whole Genome Sequencing and Bioinformatic Analysis of Aeromonas Veronii JY01 from Goldfish

维氏气单胞菌(Aeromonasveronii JY01)是从福州某养殖场内金鱼烂尾、腐皮和溃疡等病灶处分离出的1株革兰氏阴性优势菌。为了解其基因组结构及功能特征,基于IlluminaPE150和Pacbio测序平台对维氏气单肥菌菌株JY01进行全基因组测序,并对测序数据进行组装和组分分析,利用生物信息学手段进行基因预测与功能注释、同时预测致病因子和耐药基因。结果表明:维氏气单肥菌菌株JY01基因组序列长度为4.97Mb,GC含量为58.17%;共预测到4601个编码基因、31个rRNA、16个基因岛及124个tRNA;其中,3461个基因在COG中获得注释,3082个基因聚集到GO聚类分析中,4345个基因在KEGG代谢通路中富集,预测维氏气单胞菌菌株JY01携带13种毒力因子包含240个相关毒力基因及9大类抗生素耐药性包含175个抗生素耐药基因。

Aeromonas veronii JY01 is a strain of dominant gram-negative bacterium isolated from the lesions of goldfish,such as rotten tails,rot,and ulcers in a farm in Fuzhou.In this study,the whole genome of the strain of Aeromonas veronii JY01 was sequenced based on the IlluminaPE150 sequencing platform and Pacbio sequencing platform.The sequencing data was assembled and analyzed,the gene prediction and functional annotation were performed by bioinformatics methods,meanwhile the prediction of pathogenic genes and drug resistance genes were carried out.The results showed that the genomic sequence length of the strain of Aeromonas veronii JY01 was 4.97 Mb,and the GC content was 58.17%.A total of 4601 encoding genes,31 rRNA,16 gene islands and 124 tRNA were predicted,among which,3461 genes were annotated in COG,3082 genes were aggregated in GO cluster analysis,and 4345 genes were enriched in KEGG metabolic pathway.It was predicted that the strain of Aeromonas veronii JY01 carried 13 virulence factors including 240 related virulence genes,and 9 types of antibiotics resistance including 175 antibiotic resistance genes.