脂多糖构建大鼠肝内胆管炎症动物模型的浓度研究

Study on the concentration of lipopolysaccharide in constructing rat model of intrahepatic cholangitis

目的研究脂多糖(LPS)构建大鼠肝内胆管炎症动物模型的最佳浓度。方法80只大鼠分为空白对照组、模型组A、B和C,每组各20只。空白对照组按5 mL/kg在肝内胆管一次性注射生理盐水,模型组A、B、C分别按5.00、2.50、1.25 mg/kg 3种不同浓度在肝内胆管一次性注射LPS构建大鼠肝内胆管炎症动物模型,观察大鼠存活时间共7 d。分别于造模后2、4、6、8 h和7 d采取各组大鼠血液进行血常规、生化检测,观察大鼠血常规中白细胞计数(WBC)、中性粒细胞百分比(N%)、丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)炎性指标;同时采取各组大鼠肝脏组织,进行苏木素-伊红染色观察大鼠肝脏组织病理特征。根据造模结果分析LPS构建大鼠肝内胆管炎症动物模型的最佳浓度。结果在造模后2、4、6、8 h及造模后7 d,模型组C的WBC、N%、ALT及AST水平较其他3组明显升高(P<0.05)。模型组肝脏病理均有不同程度改变,其中模型组C肝脏病理组织炎性改变及肝损伤改变最为明显。结论在肝内胆管注射1.25 mg/kg LPS是构建大鼠肝内胆管炎症动物模型的最佳浓度。

Objective To investigate the optimal concentration of lipopolysaccharide(LPS)in constructing rat model of intrahepatic cholangitis.Methods A total of 80 rats were divided into the blank control group,the model group A,the model group B and the model group C with 20 rats in each group.In the blank control group,5 mL/kg sodium chloride injection was injected into intrahepatic bile duct at one time.The model group A,B,C was injected with LPS at 5.00,2.50 and 1.25 mg/kg,respectively,to establish the rat model of intrahepatic cholangitis.The survival time of the rats was observed for 7 days.Blood samples of the four groups were taken at 2,4,6,8 h and 7 d after modeling,respectively,and the blood routine and biochemical tests were performed to observe white blood cell count(WBC),percentage of neutrophils(N%),alanine aminotransferase(ALT)and aspartate transaminase(AST)inflammatory indexes.At the same time,liver tissue of rats in each group was stained with hematoxylin-eosin to observe the pathological characteristics of liver tissue.According to the modeling results,the optimal concentration of LPS to construct the rat model of intrahepatic cholangitis were evaluated.Results In the mold after 2,4,6,8 h and 7 d after building,compared with the other three groups,WBC,N%,ALT and AST levels in the model group C significantly elevated(P<0.05).Liver pathology in the model group was changed to varying degrees,among which the changes of inflammatorytissue and liver injury in the model group C were the most obvious.Conclusion Intrahepatic bile duct injection of 1.25 mg/kg LPS is the best concentration for the establishment of rat model of intrahepatic cholangitis.