苦荞CCT基因家族生物信息学及表达分析

Bioinformatics and Expression Analysis of CCT Gene Family in Fagopyrum tataricum

CCT转录因子在调控植物花期、生长发育及抗非生物胁迫等方面发挥着重要的功能。本研究以拟南芥AtCCT基因家族为参考序列,利用本地BLAST并结合保守结构域等生物信息学工具,筛选出苦荞FtCCT基因家族成员,并对其理化性质、染色体分布、基因结构、系统进化及表达水平进行分析。结果显示:从苦荞中共鉴定出35个FtCCT基因,含1-8个内含子;编码蛋白有117-753个氨基酸残基,等电点为4.96-9.51,均为亲水性蛋白。染色体定位分析表明,这些基因在8条染色体上均有分布。苦荞FtCCT基因家族含有10个保守基序和5个保守结构域,且都含有CCT保守结构域。系统进化分析表明,苦荞的FtCCT基因家族与拟南芥一样可分为3个亚家族,其中CMF亚家族的成员最多。35个FtCCT基因在苦荞根、茎、叶和花中的表达水平具有差异性,在叶和花中具有高表达量的成员较多,只有少数的成员在根和茎中高表达。本研究为进一步解析CCT基因调控苦荞花期及生长发育奠定基础。

CCT transcription factor plays an important role in the regulation of plant flowering,growth and development,as well as resistance to abiotic stress.The CCT gene family of Arabidopsis thaliana was used as the reference sequence in this study.The FtCCT gene family members of Fagopyrum tataricum were screened out by bioinformatics tools,including local BLAST and conserved domains.The physicochemical properties,chromosome distribution,gene structure,phylogenetic evolution and expression level were analyzed.The results showed that a total of 35 FtCCT genes were identified in F.tataricum where the introns ranged from 1 to 8.The encoded protein had 117-753 amino acid residues with isoelectric points of 4.96-9.51,all of which were hydrophilic proteins.Chromosomal mapping analysis showed that these genes were distributed on all 8 chromosomes.The FtCCT gene family contains 10 conserved motifs and 5 conserved domains,and all of them contained CCT conserved domains.Phylogenetic analysis showed that CCT gene family of F.tataricum could be divided into three subfamilies like A.thaliana,among which the CMF subfamily had the most members.The expression levels of 35 FtCCT genes in roots,stems,leaves and flowers of F.tataricum were different.There were more members with high expression levels in leaves and flowers,and only a few members were highly expressed in roots and stems.This study lays a foundation for further analysis of CCT gene regulation in flowering period and growth of F.tataricum.