贵州地方高粱种质资源遗传多样性分析

Genetic Diversity Analysis of Sorghum Germplasm Resources in Guizhou Province

为了解贵州省地方高粱种质资源的遗传差异和亲缘关系,对26份高粱种质进行了SSR分子标记和表型性状分析。结果表明,13对SSR引物在供试材料中共检测出26个等位基因,有效等位基因数为21.84。在遗传相似系数0.457处将26份参试材料聚为四类,供试高粱亲缘关系的远近与来源机构相关性不大。参试材料的表型性状变异系数范围在13.09%~44.46%之间,均值为22.7%,其中穗粒重存在较大程度的遗传变异,变异系数为44.46%;株高、穗长、叶片数、旗叶长和旗叶宽与穗粒重呈极显著正相关,相关系数分别为0.311、0.324、0.353、0.302、0.277,千粒重与穗粒重呈极显著正相关,相关系数为0.291;基于表型性状聚类分析将26份高粱材料分为5个类群。

To understand the genetic differences and genetic relationships of sorghum germplasm resources in Guizhou Province,SSR molecular markers and phenotypic traits of 26 sorghum germplasms were analyzed.The results showed that a total of 26 alleles were detected by 13 pairs of SSR primers,and the effective number of alleles was 21.84.The 26 samples were grouped into four groups at the genetic similarity coefficient of 0.457,and there was little correlation between the genetic relationship and the sources of the tested sorghum.The variation coefficient of phenotypic traits of the tested materials ranged from 13.09%to 44.46%,with an average of 22.7%.There was a large degree of genetic variation in spike grain weight,with a variation coefficient of 44.46%.Plant height,panicle length,leaf number,flag leaf length and flag leaf width were extremely positive correlation with panicle grain weight(correlation coefficients were 0.311,0.324,0.353,0.302 and 0.277,respectively).And,the 1000-grain weight was also very positive correlation with panicle grain weight(correlation coefficients were 0.291).Therefore,the 26 sorghum germplasms were divided into 5 groups based on cluster analysis of phenotypic traits.