血清白细胞介素-10、白细胞介素-1β与慢性乙型肝炎肝纤维化程度的关系

Relationship Between Serum Interleukin-10 and Interleukin-1β and Liver Fibrosis in Patients With Chronic Hepatitis B

目的观察血清白细胞介素(IL)-10、IL-1β在慢性乙型肝炎(CHB)患者中的表达,并分析二者与肝纤维化(HF)程度的关系。方法选取2019年3月至2021年3月商丘市第四人民医院收治的100例CHB患者为研究对象,入院时行穿刺活检,进行常规HE染色和肝组织胶原纤维Masson三重染色,参照病理结果按HF程度分为无纤维化组(S_(0)期)、轻度组(S_(1)~S_(2)期)与中重度组(S_(3)~S_(4)期);同时检测患者入院时血清IL-10、IL-1β水平,询问并记录患者一般资料;分析血清IL-10、IL-1β水平与CHB患者HF程度的关系。结果100例CHB患者经穿刺活检病理评估,无纤维化组8例,占8.00%;轻度组54例,占54.00%;中重度组38例,占38.00%。中重度组入院时血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、IL-1β水平均高于轻度组、无纤维化组,IL-10水平低于轻度组、无纤维化组,差异有统计学意义(P<0.05);无纤维化组与轻度组入院时血清ALT、AST、IL-10、IL-1β水平比较,差异无统计学意义(P>0.05)。3组其他一般资料比较,差异无统计学意义(P>0.05)。经logistic回归分析结果显示,入院时血清ALT、AST、IL-1β过表达,IL-10低表达与CHB患者HF程度有关,可能作为CHB患者HF加重的风险因子(P<0.05)。结论血清IL-10低表达、IL-1β过表达与CHB患者HF程度存在一定联系。

Objective To observe the expression of serum interleukin(IL)-10 and IL-1βin patients with chronic hepatitis B(CHB),and to analyze the relationship between them and the degree of hepatic fibrosis(HF).Methods A total of 100 patients with CHB who were treated in Shangqiu Fourth People’s hospital from March 2019 to March 2021 were selected as the research subjects.Biopsy was performed at admission,routine HE staining and Masson triple staining were performed,according to the pathological results,the patients were divided into non fibrosis group(S_(0) stage),mild group(S_(1)-S_(2) stage)and moderate to severe group(S_(3)-S_(4) stage).The serum levels of IL-10 and IL-1βof patients were detected,the baseline data of patients were inquired and recorded,the relationship between serum IL-10,IL-1βlevels and the degree of HF in CHB patients was analyzed.Results Among 100 patients with CHB evaluated by puncture biopsy pathology,8 cases were in non fibrosis group,accounting for 8.00%;54 cases were in mild group,accounting for 54.00%;38 cases were in moderate to severe group,accounting for 38.00%.The levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST)and IL-1βof moderate to severe group were higher than those of the mild group and non fibrosis group,while the level of IL-10 was lower than that of the mild group and non fibrosis group,the differences were statistically significant(P<0.05),there was no statistical significant difference in serum ALT,AST,IL-10 and IL-1βlevels at admission between non fibrosis group and mild fibrosis group(P>0.05).There was no statistical significant difference in other baseline data among the three groups(P>0.05).The logistic regression analysis results showed that the over expression of ALT,AST and IL-1βand the low expression of IL-10 were related to the degree of HF in CHB patients,which might be risk factors for HF aggravation in CHB patients(P<0.05).Conclusion The low expression of IL-10 and the over expression of IL-1βare related to the degree of HF in CHB patients.