基于毒痰瘀虚理论的中药复方对肝癌小鼠肿瘤组织的抑制作用

Objective To observe the inhibitory effect of traditional Chinese medicine compound on tumor tissue of mice with liver cancer based on the theory of toxin,thlegm,blood stasis and virtual

目的:观察基于毒痰瘀虚理论的中药复方及拆方组方对肝癌小鼠肿瘤组织及p-GSK3β、β-catenin蛋白的影响。方法:40只昆明小鼠随机分为8组:空白组、索拉菲尼组、全方组、脾虚组、肾虚组、肾阳虚组、肾阴虚组、毒痰瘀组,构建H22肝癌荷瘤小鼠模型,每组小鼠予以相应药物灌胃,连续给药2周后,比较肿瘤抑制率,以免疫组化法分析瘤体内微血管密度(MVD),Western blot法检测肿瘤组织中p-GSK3β、β-catenin蛋白的表达。结果:肿瘤体积比较,各中药组小鼠肿瘤体积均小于空白组(P<0.01),全方组、脾虚组小鼠肿瘤体积无明显差异,次于索拉菲尼组,优于其他拆方组(P<0.05);MVD值比较,各中药组小鼠MVD值均低于空白组(P<0.01),全方组、脾虚组、肾虚组、肾阳虚组小鼠MVD值均低于毒痰瘀组(P<0.05);p-GSK3β、β-catenin蛋白表达量比较,各中药组小鼠p-GSK3β、β-catenin蛋白表达量低于空白组(P<0.01或P<0.05),肾虚组小鼠β-catenin蛋白除外,全方组小鼠p-GSK3β蛋白表达量低于其他拆方组方组(P<0.01或P<0.05),索拉菲尼组与全方组小鼠p-GSK3β蛋白表达无明显差异(P>0.05),毒痰瘀组小鼠p-GSK3β蛋白表达量高于其他各中药组(P<0.05)。结论:基于毒痰瘀虚理论的中药复方及其拆方组方均可显著抑制H22肝癌小鼠瘤体及微血管的生长、降低p-GSK3β、β-catenin蛋白的表达,在抑制p-GSK3β、β-catenin蛋白表达方面全方效果更好,其不同拆方组方在抗癌疗效方面有所侧重。

Objective:To observe the effects of traditional Chinese medicine(TCM)and its disassembled prescriptions based on the theory of toxin,thlegm,blood stasis,and virtual on tumor tissue and p-GSK3β、β-catenin protein in mice with liver cancer.Methods:Forty Kunming mice were randomly divided into eight groups:blank group,sorafenib group,whole prescription group,spleen deficiency group,kidney deficiency group,kidney yang deficiency group,kidney yin deficiency group,and toxic phlegm and blood stasis group.The mice in each group were given corresponding drugs by gavage.After two weeks of continuous administration,the tumor inhibition rate was compared,the microvessel density(MVD)in the tumor was analyzed by immunohistochemistry,and western blot was used to detect the expression of p-GSK3β、β-catenin in tumor tissues.Results:Compared with the blank group,the tumor volume of each traditional Chinese medicine group was smaller than that of the blank group(P<0.01),and there was no significant difference in the tumor volume between the whole prescription group and the spleen deficiency group,which was inferior to sorafenib group and superior to other disassembled prescription groups(P<0.05).The MVD value of each Chinese medicine group was lower than that of the blank group(P<0.01),and that of the whole prescription group,spleen deficiency group,kidney deficiency group,and kidney yang deficiency group was lower than that of toxic phlegm and blood stasis group(P<0.05).Compared with the expression of p-GSK3βandβ-catenin,the protein expression of p-GSK3βandβ-catenin in each Chinese medicine group was lower than that in the blank group(P<0.01 or P<0.05);except forβ-catenin protein in the kidney deficiency group,the protein expression of p-andβ-catenin in the whole prescription group was lower than that in the other decoction groups(P<0.01 or P<0.05),and there was no significant difference in the expression of p-GSK3βbetween sorafenib group and the whole prescription group(P>0.05).The expression of p-GSK3βprotein in the blood stasis group was higher than that in other traditional Chinese medicine groups(P<0.05).Conclusion:Based on the theory of toxic phlegm and blood stasis,the traditional Chinese medicine compound,and its disassembled prescriptions can significantly inhibit the growth of tumor body and microvessel,and reduce the expression of p-GSK3βandβ-Catenin protein in H22 hepatoma mice.In terms of inhibiting the expression of p-GSK3βandβ-Catenin,the whole prescription has a better effect,and its different disassembled prescriptions have some emphasis on anti-cancer effects.