基于质谱的蛋白质组学方法新进展

Recent Progress of Analytical Methods of Proteomics Based on Mass Spectrometry

蛋白质组学是后基因组时代的科研热点,其研究方向主要涵盖蛋白质表达谱、蛋白质翻译后修饰谱、蛋白质相互作用谱和单细胞蛋白质组等定性分析以及相对和绝对定量分析,现已广泛应用于生物、医药及病理研究中。由于质谱具有灵敏、准确、高通量等特点,是蛋白质组学研究必不可少的工具。本文综述了近年来基于质谱技术的蛋白质组鉴定及定量方法,并展望未来蛋白质组学研究的发展方向。

Proteomics is widely used in biological,pharmaceutical and pathological areas as a hot research topic in the post-genome era.The research areas of proteomics mainly cover qualitative analysis,such as protein expression profiles,protein post-translational modifications,protein-protein interactions,single cell proteomes,as well as proteome relative and absolute quantitative analysis.Mass spectrometry is an essential tool in proteome researches because of its sensitivity,accuracy and high throughput.In this paper,the identification and quantitative methods of proteomics based on mass spectrometry were reviewed,and the development direction of proteomics in the future was also prospected.