灵武长枣嫁接和根蘖繁殖植株果实转录组差异分析

Transcriptome Differences Analysis of Fruit of Grafting and Root Tiller Propagation of ’Lingwuchangzao’ Jujube

为解析嫁接繁殖和根蘖繁殖灵武长枣果实糖和酸转化差异,本研究以两种繁殖方式的成熟期果实为材料,利用高通量测序平台Illumina/Nova-Seq 2500进行了转录组测序,对转录组数据质量评估获得两样品的序列长度分别为24.48 Gb (嫁接)和28.64 Gb (根蘖),错误率为0.03%,GC含量44.11%~44.56%,Q30大于90.54%,转录组数据与冬枣基因组序列比对,所有的转录组Reads均分布于冬枣12条染色体上,基因定位的Reads数量嫁接繁殖比根蘖繁殖多;差异表达基因分析筛选到27个显著差异表达基因,其中9个显著上调,18个显著下调;GO富集分析表明15个差异基因显著富集到GO的三大功能类别分子功能、细胞组分、生物过程中;KEGG富集分析表明10个差异表达基因显著富集到12条代谢通路中,其中包括3个上调基因和7个下调基因;进一步筛选到调控糖及有机酸代谢关键差异基因BAM1、PCO和HAOX1在淀粉和蔗糖代谢、牛胆素和亚牛磺酸代谢、乙醛酸和二羧酸代谢中显著表达,并在糖和有机酸的相互转化中起着关键调控作用。本研究表明嫁接繁殖和根蘖繁殖灵武长枣果实在糖和有机酸代谢存在差异,为灵武长枣优质果品繁育提供理论基础。

In order to reveal the difference of fruit sugar and organic acid transformation between grafting propagation(YJ) and root tiller propagation(YG) of ’Lingwuchangzao’ transcriptome sequencing of two kinds of propagation fruits in mature stage using Illumina/Nova-Seq 2500 platform. The sequence length of the two samples was24.48 Gb(YJ) and 28.64 Gb(YG) respectively, and the error rate was 0.03%, the GC content was 44.11%~44.56%,the Q30 was more than 90.54%. Differential expression gene analysis screened 27 significantly differentially expressed genes, of which 9 were significantly up-regulated and 18 were significantly down-regulated;GO enrichment analysis showed that 15 differentially expressed genes were significantly enriched into GO three-generation functional class including molecular functions, cell components, biological processes;KEGG enrichment analysis showed that 10 differentially expressed genes were significantly enriched into 12 metabolic pathways, including3 up-regulated genes and 7 down-regulated genes;Key differential genes regulating sugar and organic acid metabolism were screened and gene BAM1, PCO and HAOX1 were found to be significantly expressed in starch and sucrose metabolism, taurine and hypotaurine metabolism, and glyoxylate and dicarboxylate metabolism. This study showed that there are differences in sugar metabolism and organic acid metabolism between the fruit of grafting and root tiller propagation, which provided theoretical basis for high quality fruit breeding.