利用SSR标记鉴定杂交粳稻‘花优14’种子纯度

Identification of Seed Purity on Hybrid Japonica Rice ’Huayou14’ Using SSR Markers

本研究以杂交粳稻‘花优14’及其亲本为材料,利用SSR分子标记技术,对其多态性进行了引物筛选和种子纯度鉴定研究。从48对广泛分布在水稻12条染色体上的候选SSR分子标记中,筛选得到6对引物,在‘花优14’和其双亲之间表现为稳定的共显性且能将杂交种及其亲本完全区分,利用这些引物对‘花优14’种子进行分子纯度鉴定,并将鉴定结果与田间小区种植纯度鉴定结果相比较,结果显示两者鉴定结果基本一致。因此,最终确定了多态性位点最多且特异性差异最显著的RM274和RM1195标记组合作为核心引物,建立了一套更为快速、有效地鉴定杂交粳稻‘花优14’种子纯度的技术方法。

In this st udy, t he hybrid japonica rice ’Huayou14’ and its parents were used as materials, using SSR molecular marker technology, to select primers and identify seed purity according to its polymorphism. From 48 pairs of candidate SSR molecular markers widely distributed on 12 rice chromosomes, 6 pairs of primers were screened, which showed stable codominance between the hybrid and its parents and could completely distinguish the hybrid line and its parents. Using these primers to carry out molecular purity identification of ’Huayou14’ seeds,and comparing the identification results with the field planting purity identification results, the results showed that the identification results of the two were basically the same. Finally, the RM274 and RM1195 marker combinations with the most polymorphic sites and the most significant differences in specificity were finally determined as core primers, and a more rapid and effective technical method for identifying the seed purity of hybrid japonica rice ’Huayou14’ was established.