基于SLAF-seq技术连翘SNP分子标记开发及遗传多样性分析

SNP Molecular Markers Development and Genetic Diversity Analysis of Forsythia suspensa Based on SLAF-seq Technology

连翘(Forsythia suspensa (Thunb.) Vahl)是一种极具开发价值的药用植物,本研究利用特异性位点扩增片段测序技术对39份连翘种质资源进行了分子标记开发,通过测序共获得112.28 Mb reads数据,各样本reads数据在1 324 860~5 911 565之间,样本平均测序质量值Q30为96.29%;平均GC含量为36.97%。通过生物信息学分析,本研究获得535 357个SLAF标签,样本的平均测序深度为16.20倍,其中多态性的SLAF标签共有262 297个,开发获得1 809 741个SNP标记。利用开发的SNP分子标记将39份连翘种质资源分为4组,连翘SNP分子标记的研究可为连翘种质资源鉴定和遗传多样性分析提供理论基础。

Forsythia suspensa(Thunb.) Vahl is an important medicinal plant that has great value to study. In this study, the 39 Forsythia suspensa materials were used specific loci amplified fragment sequencing technology(SLAF-seq) to develop SNP molecular markers. A total of 112.28 Mb reads data were obtained by sequencing. The reads data of each sample ranged from 1 324 860~5 911 565. The average sequencing quality value(Q30) and GC content of samples was 96.29% and 36.97%, respectively. Analysis of bioinformatics, there were 535 357 SLAF tags, in which 262 297 SLAF tags were polymorphic, and the average sequencing depth of the samples was 16.20 times. A total of 1 809 741 SNP molecular markers were obtained and 39 Forsythia suspensa materials were divided into 4 groups. This study could provide theoretical basis for germplasm resource identification and genetic diversity analysis of Forsythia suspensa.