3种抗抑郁中药方作用比较研究

The Comparison of Three Traditional Chinese Medicine Formulas on Their Antidepressant Efficacy

目的比较3个中药处方的抗抑郁作用,筛选出抗抑郁效果最好的处方。方法ICR雄性小鼠(130只)随机分为对照组,模型组,阿米替林组,帕罗西汀组,处方一高、中、低浓度组,处方二高、中、低浓度组,处方三高、中、低浓度组,每组10只。通过采用小鼠悬尾实验、强迫游泳实验以及建立小鼠利血平拮抗模型,检测其眼睑下垂度、体温和自主活动能力,以此检测小鼠的抑郁样行为。结果预防性给药7 d后,与对照组相比,阳性药及3个处方均能不同程度缩短小鼠悬尾不动时间(P<0.05,P<0.01);阿米替林、处方二能显著缩短强迫游泳不动时间(P<0.05),增加游泳距离(P<0.01),但处方一和三无效。预防给药14 d后,与对照组相比,各阳性药与3个处方均能显著缩短小鼠悬尾不动时间(P<0.05,P<0.01);同时,各阳性药与处方一和处方二能明显缩短小鼠强迫游泳不动时间(P<0.05,P<0.01),增加小鼠游泳距离(P<0.05),但处方三无效。在利血平拮抗实验中,与对照组相比,模型组运动时间明显减少(P<0.01),体温出现明显下降(P<0.01),小鼠眼睑下垂度明显增加(P<0.01);与模型组相比,阿米替林、帕罗西汀以及3个处方均能不同程度逆转利血平所致小鼠运动不能(P<0.05),体温下降(P<0.05,P<0.01),眼睑下垂(P<0.05,P<0.01)的现象。结论3个中药方均具有一定的抗抑郁作用,其中处方二的药理作用更广,抗抑郁作用效果更好,表现为起效时间短、药效强。

Objective To compare the effects of three traditional Chinese medicine(TCM)formulas on their antidepressent efficacy and to screen out the prescription with the best antidepressant effect.Methods 130 ICR male mice were randomly divided into 13 groups,including the control group,the model group,the amitriptyline group,the paroxetine group,the first formula groups(high,medium and low dosages),the second formula groups(high,medium and low dosages),the third formula groups(high,medium and low dosages).10 mice in each group.Tail suspension test,forced swimming test and reserpine induced depression mice model were used.The degree of eyelid ptosis,body temperature and autonomous activity were measured to test the depressive-like behavior in mice.Results Prophylaxis and treatment of TCM formulas for 7 days,compared with the control group,the positive medicine and the three formulas significantly shorten the immobility time in the tail suspension test in mice(P<0.05,P<0.01);amitriptyline and the second formula significantly shorten the immobility time of forced swimming(P<0.05)and increased swimming distanse(P<0.01).The first formula and third formula had no effect in the forced swimming test in mice.Prophylaxis and treatment of TCM formulas for 14 days,compared with the control group,the positive medicines and the three formulas significantly shorten the immobility time in the tail suspension test(P<0.05,P<0.01).At the same time,positive medicines and the first and the second formulas significantly shorten the immobility time of forced swimming(P<0.05,P<0.01)and increased swimming distanse(P<0.05),but the third formula had no effect in the forced swimming test in mice.In the reserpine antagonistic experiment,compared with the control group,the movement time of the model group was significantly reduced(P<0.01),the body temperature was significantly decreased(P<0.01),and the ptosis degree of the mouse eyelid was significantly increased(P<0.01).Compared with the model group,amitriptyline,paroxetine and three prescriptions could reverse the locomotor failure(P<0.05),body temperature decreased(P<0.05,P<0.01),eyelid ptosis(P<0.05,P<0.01)induced by reserpine to varying degrees.Conclusion Three TCM formulas have effects on antidepression,but the second formula is the best one which has fast and strong effects on antidepression compared to the other two formulas.