Zea-longa评分与改良Garcia评分应用于针刺治疗CIRI大鼠神经功能缺损评估的研究

Evaluation Research of Zea-longa Score and Modified Garcia Score Applied to Acupuncture Treatment of Neurological Deficits in CIRI Rats

目的比较Zea-longa评分与改良Garcia评分用于针刺治疗脑缺血再灌注损伤(cerebral ischemia reperfusion injury,CIRI)大鼠的神经功能缺损评估的稳定性、准确性。方法将60只SPF级SD大鼠随机分为假手术组、模型组、针刺组,每组20只。采用线栓法制备大鼠大脑中动脉闭塞模型,2 h后,将线栓拔出1 cm左右,建立CIRI模型。针刺组针刺“大椎”“百会”“水沟”,每12 h进行1次,共治疗7次。72 h后,分别采用Zea-longa评分与改良Garcia评分进行评价,每组选取10只大鼠行TTC染色,并测定脑梗死面积;分别分析和比较Zea-longa评分和改良Garcia评分与脑梗死面积的相关性。结果CIRI术后,大鼠均表现有一定程度神经功能的缺损症状,脑组织TTC染色可见脑梗死病灶。针刺治疗能显著减少脑梗死面积(P<0.05),并改善神经功能(P<0.05,P<0.01)。相关性分析发现,改良Garcia评分与脑梗死面积的r值为-0.647(P<0.01),而Zea-longa评分与脑梗死面积的r值为0.575(P<0.01);针刺与改良Garcia评分及脑梗死面积三者相关性分析r值为-0.522(P<0.05);而针刺与Zea-longa评分及脑梗死面积r值为0.495(P<0.05)。结论改良Garcia评分与Zea-longa评分均可用于CIRI评价研究及针刺疗效评估。在对CIRI大鼠进行感觉功能相关研究时可优先采用改良Garcia评分,偏向运动功能评估时可考虑与Zea-longa评分联合使用,能够比较全面地反映模型大鼠神经功能损伤的严重程度和治疗后的神经功能的恢复程度。

Objective To compare the stability and accuracy of the Zea-longa score and the modified Garcia score for the assessment of neurological deficits in rats with acupuncture treatment of cerebral ischemia reperfusion injury(CIRI).Methods 60 SPF SD rats were randomly divided into sham-operation group,model group,and acupuncture group,with 20 rats in each group.The rat middle cerebral artery occlusion model was established by suture-occluded method.After 2 hours,the suture was pulled out about 1 cm to establish the CIRI model.In the acupuncture group,“Dazhui”(DU14),“Baihui”(DU20)and“Shuigou”(DU26)were performed for 12 hours/time for a total of 7 treatments.After 72 hours,Zea-longa score and modified Garcia score were used for evaluation.10 rats in each group were selected to perform TTC staining and measure cerebral infarction area;the correlation between Zea-longa score and modified Garcia score and cerebral infarction area was analyzed and compared.Results After CIRI,the rats all showed signs of neurological deficits to a certain degree.Cerebral infarction lesions could be seen by TTC staining of brain tissue.Acupuncture treatment can significantly reduce the area of cerebral infarction(P<0.05)and improve nerve function(P<0.05,P<0.01).Correlation analysis found that the r value of the modified Garcia score and cerebral infarction area was-0.647(P<0.01),and the r value of Zea-longa score and cerebral infarction area was 0.575(P<0.01);the r value of the correlation analysis between acupuncture and modified Garcia score and cerebral infarction area was-0.522(P<0.05);while the r value of acupuncture and Zea-longa score and cerebral infarction area was 0.495(P<0.05).Conclusion Both the modified Garcia score and Zea-longa score can be used for CIRI evaluation research and acupuncture efficacy evaluation.When conducting sensory function-related research in CIRI rats,the modified Garcia score can be used first,and the Zea-longa score can be considered when biased in motor function assessment,but the combination of the two can more fully reflect the severity of nerve function injury in model rats and the recovery degree of nerve function after treatment.