牙龈上皮β-防御素-2诱导表达的中药筛选

Screening of Chinese herbal medicine for inducing expression of β-defensin-2 in gingival epithelium

目的筛选出对大鼠牙龈上皮β-防御素-2具有诱导表达作用的中药。方法2019年2月至2020年4月选取90只大鼠作为研究对象。将90只健康成年Wistar大鼠随机分为固有表达组(10只)、阴性对照组及阳性对照组(各10只);实验组分别给予药物(黄芪组、板蓝根组、丹参组、柴胡组、鱼腥草组、穿心莲内酯组,每组10只,共60只),连续给药7 d后将大鼠处死,切取大鼠牙龈上皮,采用免疫组织化学方法检测大鼠牙龈上皮β-防御素-2蛋白的表达情况。结果黄芪组和板蓝根组大鼠牙龈上皮β-防御素-2蛋白的表达明显高于固有表达组,差异有统计学意义(P<0.05),但与阳性对照组比较,差异无统计学意义(P>0.05)。结论中药黄芪和板蓝根能够诱导大鼠牙龈上皮β-防御素-2的表达增高,黄芪和板蓝根具有调节大鼠口腔黏膜天然免疫的作用。

Objective To screen out the Chinese herbal medicine which can induce the expression of β-defensin-2 in rat gingival epithelium.Methods 90 rats in our hospital from February 2019 to April 2020 were selected as the research objects.Ninety healthy adult Wistar rats were randomly divided into three groups:the inherent expression group(10 rats),the negative control group(10 rats)and the positive control group(10 rats).The experimental group were given drugs respectively(the astragalus membranaceus group,the Radix Isatidis group,the salvia miltiorrhiza group,the radix bupleuri group,the herba houttuyniae group,and the andrographolide lactone group,with 10 rats in each group,a total of 60 rats).After 7 days of continuous administration,the rats were sacrificed,and the gingival epithelium of the rats was cut.The expression of β-defensin-2 protein in the gingival epithelium of the rats was detected by the immunohistochemical method.Results The expressions of β-defensin-2 protein in the gingival epithelium of rats in the astragalus membranaceus group and the radix isatidis group were significantly higher than that in the inherent expression group,and the differences were statistically significant(P<0.05),but no statistically significant differences were observed compared with the positive control group(P>0.05).Conclusion Astragalus membranaceus and radix isatidis can induce the increase of β-defensin-2 expression in the gingival epithelium of rats,suggesting that astragalus membranaceus and radix isatidis can regulate the innate immunity of oral mucosa of rats.