子宫颈鳞癌AIM2和血清SCC表达与预后相关性分析

Relationship between Tissue AIM2 and Serum SCC Expression and the Prognosis of Cervical Squamous Cell Carcinoma

目的:探究子宫颈鳞癌患者黑色素瘤缺乏因子2(AIM2)与血清鳞状上皮细胞癌相关抗原(SCC)表达水平及其临床意义。方法:选择2013年6月1日至2015年6月30日于徐州市中心医院妇科接受手术治疗的80例子宫颈鳞癌患者的肿瘤组织及30例正常子宫颈患者组织,应用免疫组化检测上述标本中AIM2的表达水平,回顾性收集子宫颈鳞癌患者临床病理参数及其术前血清SCC水平,分析两种指标与临床病理特征的关系。结果:①子宫颈鳞癌组织AIM2高表达率明显高于正常子宫颈组织(68.8%vs 26.7%,P<0.001),子宫颈鳞癌组织AIM2表达水平与术前血清SCC水平呈正相关(r=0.275,P=0.013);②AIM2表达与肿瘤的肿瘤直径、病理学分级、FIGO分期、肌层浸润深度及淋巴结转移有关(P<0.05);术前SCC水平与肿瘤的病理学分级、FIGO分期、肌层浸润深度及淋巴结转移有关(P<0.05);③AIM2高表达患者总生存率(OS)(56.1%)明显低于低表达患者(92.0%),差异有统计学意义(P=0.002)。术前SCC阳性患者OS(53.9%)明显低于阴性患者(87.5%),差异有统计学意义(P=0.002);④多因素COX回归分析结果显示,AIM2表达、术前SCC水平是子宫颈鳞癌患者预后的独立危险因素。结论:子宫颈鳞癌组织中AIM2过表达与患者不良预后有关,联合分析子宫颈鳞癌组织AIM2及血清SCC有助于评估子宫颈鳞癌患者预后。

Objective:To investigate the expression of absent in melanoma 2(AIM2)and serum squamous cell carcinoma antigen(SCC)in patients with cervical squamous cell carcinoma.Methods:Tumor tissues from 80 cervical squamous cell carcinoma patients who underwent surgery at the gynecological department of Xuzhou Central Hospital from June 1,2013 to June 30,2015 and 30 normal uterine cervical tissues were collected.The expression of AIM2 was detected by immunohistochemistry.Clinicopathological parameters of patients with cervical squamous cell carcinoma and their preoperative serum SCC levels were retrospectively collected.The relationships between the two indexes and clinicopathological characteristics were analyzed.Results:①The high expression rate of AIM2 in cervical squamous cell carcinoma tissue was significantly higher than that in normal cervical tissue(68.8%vs 26.7%,P<0.001).The expression level of AIM2 in tumor tissue of cervical squamous cell carcinoma patients was positively correlated with the preoperative serum SCC level(r=0.275,P=0.013).②AIM2 expression was related to tumor diameter,pathological grade,FIGO staging,depth of myometrial invasion and lymph node metastasis(P<0.05),preoperative SCC level was related to tumor pathological grade,FIGO staging,the depth of myometrial invasion and lymph node metastasis(P<0.05).③OS of patients with high AIM2 expression(56.1%)was significantly(P=0.002)lower than that of patients with low expression(92.0%).The OS(53.9%)of patients with positive SCC before surgery was significantly(P=0.002)lower than that of patients with low expression(87.5%).④Multivariate COX regression analysis showed that AIM2 expression and preoperative SCC levels were the independent risk factors for the prognosis of patients with cervical squamous cell carcinoma.Conclusions:The overexpression of AIM2 in cervical squamous cell carcinoma is associated with poor prognosis.Combined analysis of AIM2 in cervical squamous cell carcinoma and serum SCC level is helpful to evaluate the prognosis of patients with cervical squamous cell carcinoma.