痛风性关节炎患者HLA-B^(*)5801基因型携带率及相关实验室指标分析

HLA-B^(*)5801 gene carrying rate and laboratory testing indicators in patients with gouty arthritis

目的探讨痛风性关节炎(GA)患者HLA-B^(*)5801基因型的携带情况及其与相关实验室指标的关系。方法选取120例GA患者,检测HLA-B^(*)5801基因型及尿酸(UA)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、C反应蛋白(CRP)、中性粒细胞百分比、淋巴细胞百分比、嗜酸性粒细胞百分比(EO%)、平均血小板体积(MPV)、红细胞沉降率(ESR),计算中性粒细胞/淋巴细胞比值(NLR)及ALT/ALP比值。以50名体检健康者作为正常对照组。根据UA水平将120例GA患者分为低水平UA(UA<420μmol/L)组、中水平UA(UA为420~600μmol/L)组、高水平UA(UA>600μmol/L)组。采用Correl函数分析UA与其他项目之间的相关性。结果GA患者HLA-B^(*)5801基因型携带率为29.2%。GA组UA、NLR、EO%、CRP、ESR、ALT/ALP比值均高于正常对照组(P<0.05),MPV低于正常对照组(P<0.05)。HLA-B^(*)5801基因型携带组EO%、MPV高于非携带组(P<0.05),UA低于非携带组(P<0.05);2个组之间NLR、CRP、ESR、ALT/ALP比值差异均无统计学意义(P>0.05)。低水平UA组、中水平UA组、高水平UA组ALT/ALP比值依次升高(P<0.05);高水平UA组EO%高于中水平UA组(P<0.05),NLR和ESR高于中水平UA组和低水平UA组(P<0.05);3组之间MPV、CRP差异均无统计学意义(P>0.05)。NLR、ESR、ALT/ALP比值与UA均呈正相关(r值分别为0.959、0.953、0.994,P<0.05),MPV与UA呈负相关(r=-0.998,P<0.05),EO%、CRP与UA均无相关性(r值分别为0.647、0.091,P>0.05)。结论GA患者HLA-B^(*)5801基因型携带率较高。检测HLA-B^(*)5801基因型及相关实验室指标有助于综合评估GA患者的疾病状态。

Objective To investigate the correlation of HLA-B^(*)5801 gene carrying status and laboratory testing indicators in patients with gouty arthritis(GA).Methods Totally,120 GA patients were enrolled to determine HLA-B^(*)5801 genotype,uric acid(UA),alanine aminotransferase(ALT),alkaline phosphatase(ALP),C-reactive protein(CRP),the percentage of neutrophil,the percentage of lymphocyte,the percentage of eosinophil(EO%),mean platelet volume(MPV)and erythrocyte sedimentation rate(ESR),and the neutrophil/lymphocyte ratio(NLR)and ALT/ALP ratio were calculated.A total of 50 healthy subjects were enrolled as healthy control group.According to the levels of UA,120 GA patients were classified into low-level UA(UA<420μmol/L)group,medium-level UA(UA 420-600μmol/L)group and high-level UA(UA>600μmol/L)group.The correlation between laboratory testing indicators with UA was evaluated by Correl analysis.Results Among the 120 patients,35(29.2%)cases carried HLA-B^(*)5801 gene,the UA,NLR,EO%,CRP,ESR and ALT/ALP ratio in GA group were higher than those in healthy control group(P<0.05),and MPV was lower(P<0.05).Compared with HLA-B^(*)5801 gene carrying group,EO%and MPV were higher than those in non-carrying group(P<0.05),while UA was lower than that in non-carrying group(P<0.05),and NLR,CRP,ESR,ALT/ALP ratio had no statistical significance(P>0.05).ALT/ALP ratios were increased in turn among low-level,medium-level and high-level UA groups(P<0.05).EO%in medium-level UA group was lower than that in high-level UA group(P<0.05),and NLR and ESR were higher than those in high-level UA group and low-level UA group(P<0.05).MPV and CRP had no statistical significance among the 3 groups(P>0.05).NLR,ESR and ALT/ALP ratio were positively correlated with UA(r values were 0.959,0.953 and 0.994,respectively,P<0.05),while MPV was negatively correlated with UA(r=-0.998,P<0.05).There was no correlation between EO%,CRP and UA(r values were 0.647 and 0.091,respectively,P>0.05).Conclusions The gene carrying rate of HLA-B^(*)5801 gene in patients with GA is high.The determination of HLA-B^(*)5801 gene and related laboratory testing indicators will be helpful to comprehensively evaluate the disease status of patients.