c-Myc基因启动子甲基化在类风湿关节炎中的研究

Investigation into the DNA methylation of c-Myc Gene in rheumatoid arthritis

目的探讨c-Myc基因启动子甲基化在类风湿关节炎发病中的作用。方法选取类风湿关节炎活动期患者18例,稳定期患者25例,健康对照组20例。用焦磷酸测序法检测外周血c-Myc基因启动子DNA甲基化率,用ELISA法检测外周血白介素(IL)-17水平。结果c-Myc(chr8:127736502)甲基化率在类风湿关节炎活动期、稳定期及健康对照组中差异有统计学意义(P<0.05),健康对照组与类风湿关节炎活动期和稳定期组差异均有统计学意义(均P<0.05).IL-17在类风湿关节炎活动期、稳定期及健康对照组中差异有统计学意义(P<0.05).c-Myc(chr8:127736502)甲基化率与IL-17呈负相关性(r=-0.186,P<0.05)。结论c-Myc基因低甲基化可能与类风湿关节炎的发病相关,且c-Myc(chr8:127736502)甲基化率与IL-17水平呈负相关。

Objective To investigate the role of DNA methylation of c-Myc gene in the pathogenesis of rheumatoid arthritis,the correlation between methylation rate of c-Myc gene and interleukin-17(IL-17)level,and the correlation between these two variables and inflammation indicators.Methods 18 patients with active rheumatoid arthritis,25 patients with stable stage and 20 healthy controls were selected.The pyro sequencing method was used to detect the DNA methylation rate of c-Myc gene in peripheral blood and the Elisa method was used to detect the level of IL-17 in the peripheral blood.Results The methylation rate of c-Myc(chr8:127736502)was compared in the active stage,stable stage of rheumatoid arthritis and healthy control group,and there were statistical differences(P<0.05);while,compared with healthy control group,the active stage and the stable stage of rheumatoid arthritis were statistically significant(P<0.05).IL-17 levels in the active stage,stable stage of rheumatoid arthritis and healthy controls were statistically different(P<0.05).The methylation rate of c-Myc(chr8:127736502)was negatively correlated with IL-17(r=0.186,P<0.05).Conclusion The hypomethylation of c-Myc gene might be involved in the pathogenesis of rheumatoid arthritis and the methylation rate of c-Myc(chr8:127736502)was negatively correlated with the level of IL-17.