尿中锰测定的直接稀释-电感耦合等离子体质谱法

Determination of manganese in urine by direct dilution-inductively coupled plasma mass spectrometry

目的建立直接稀释-电感耦合等离子体质谱法测定尿中锰的方法。方法以1%硝酸溶液为稀释剂,采用单因素轮换实验确定尿液的稀释倍数和内标元素,分析尿中锰测定的直接稀释-电感耦合等离子体质谱法的线性范围、相关系数、精密度、准确度和检出限。结果该方法测定尿中锰含量的线性范围为0.0~20.0μg/L,相关系数为0.9999,检出限为0.02μg/L,样品加标回收率为84.65%~103.40%,相对标准偏差为0.26%~8.17%。结论该法操作简单、灵敏度高、检出限低,可以与其他元素同时进行检测,适用于职业接锰作业工人及普通人群尿锰的测定。

Objective To establish a direct dilution-inductively coupled plasma mass spectrometry method for the determination of manganese in urine.Methods Using 1%nitric acid solution as diluent,the urine dilution factor and internal standard elements were determined by single factor rotation experiment.The linear range,correlation coefficient,precision,accuracy and detection limit of the direct dilution-inductively coupled plasma mass spectrometry for the determination of manganese in urine were evaluated.Results The linear range of this method was 0.0-20μg/L,the correlation coefficient was 0.9999,the detection limit was 0.02μg/L,the recoveries were 84.65%-103.40%,the relative standard deviations were 0.26%-8.17%.Conclusion This method has the advantages of simple operation,high sensitivity and low detection limit.It can be used for the determination of urine manganese at the same time with other elements.It is suitable for the determination of urine manganese in workers and ordinary people.