尿中钴测定的正交设计优化石墨炉原子吸收光谱法

Optimizing experimental conditions of graphite furnace atomic absorption spectrometer using orthogonal design for determination of cobalt in urine

目的选择和优化石墨炉原子吸收光谱法测定尿中钴的条件,建立尿中钴测定方法。方法于2020年4月,运用数理统计方法,对基体改进剂和光谱波长进行选择,优化石墨炉升温程序,对正交试验结果进行方差分析,工作曲线外标法定量。结果磷酸氢二铵为基体改进剂,波长240.7 nm,优化石墨炉升温程序测定显示,在5~80 μg/L呈良好的线性关系,相关系数0.999 1。方法检测限为0.9 μg/L,最低检出浓度为1.8 μg/L,回收率为94.0%~101.4%,精密度为2.6%~5.8%。结论正交设计法可高效、科学优化石墨炉原子吸收光谱各因素水平的较优组合,基于此建立的测定方法精密度高、准确度好,可适用于尿中钴的测定。

Objective To select and optimize the conditions of urine cobalt determination by graphite furnace atomic absorption spectroscopy and establish a method for urine cobalt determination.Methods In April 2020,the matrix modifier and spectrum wavelength were selected by mathematical statistics method,the heating procedure of graphite furnace was optimized,the variance of orthogonal test results was analyzed,and the working curve was quantified by external standard method.Results The results showed that the diammonium hydrogen phosphate was a matrix modifier with a wavelength of 240.7 nm,and the optimized graphite furnace heating procedure showed a good linear relationship at 5-80μg/L.The correlation coefficient was 0.9991.The detection limit was 0.9μg/L,the lowest detection concentration was 1.8μg/L.The recovery was 94.0%-101.4%,and the precision was 2.6%-5.8%.Conclusion The orthogonal design method can efficiently and scientifically optimize the optimal combination of the various factor levels of graphite furnace atomic absorption spectrometry.The determination method established based on this has high precision and good accuracy,and can be applied to the determination of cobalt in urine.