铜绿假单胞菌T6SS的组装、分泌、功能和调控

Assembly,secretion,function,and regulation of T6SS in Pseudomonas aeruginosa

作为一种对抗真核细胞和原核细胞的强有力细菌武器,VI型分泌系统(type VI secretion system,T6SS)广泛存在于革兰氏阴性菌中。铜绿假单胞菌是一种对多种抗生素具有耐药性并能够在人体引发急性和慢性感染的条件致病菌,它编码3套独立的T6SS,分别为H1-、H2-和H3-T6SS。T6SS通过介导细菌间竞争、生物被膜的形成、金属离子的摄取以及与真核宿主细胞之间的相互作用,对铜绿假单胞菌在毒力和适应环境方面发挥重要作用。本文主要对铜绿假单胞菌T6SS的组装、效应蛋白的分泌、功能及调控机制展开综述,旨在为T6SS的研究提供一定的参考,并为铜绿假单胞菌感染的预防和治疗提供一定的指导。

The type VI secretion system(T6SS)is a versatile bacterial weapon,which is widespread in Gram-negative bacteria and used to act against both eukaryotic and prokaryotic cells.Pseudomonas aeruginosa is an opportunistic pathogen that is resistant to a variety of antibiotics and can cause acute and chronic infections in humans.P.aeruginosa encodes three separate T6SSs,namely H1-,H2-,and H3-T6SS,which play key roles in virulence and environmental adaptation by mediating inter-bacterial competition,biofilm formation,metal uptake,and interaction with eukaryotic host cells.This article reviews the latest progress in assembly,secretion,function,and regulation mechanisms of T6SS in P.aeruginosa,aiming to provide reference for the study of T6SS and provide guidance for the prevention and treatment of P.aeruginosa infection.