豹猫转录组从头组装及组织特异性表达分析

De novo Transcriptome and Tissues-Specific Expression Analysis of Prionailurus bengalensis

豹猫Prionailurus bengalensis是亚洲分布最广的食肉动物之一,国家二级重点保护野生动物。本研究对豹猫6个组织(大脑、心脏、肾脏、肝脏、肺和骨骼肌)进行了转录组测序,共获得51.4 Gb的原始数据。使用Trinity进行从头组装,最终获得了369246条转录本,其平均长度为1465 bp,转录本N50长度为2660 bp,组装质量较高。注释结果显示,约42.44%(114517条)的转录本在4个公共数据库中成功获得注释,65895条转录本被分配到了386条KEGG通路中。根据转录本表达量构建各组织的表达图谱、计算组织特异性指数(TSI),39.65%的转录本为0.15≤TSI≤0.85,具有中等组织特异性,60.34%的转录本TSI>0.85,具有较高的组织特异性。统计豹猫每个组织中表达量最高的10条转录本,共39条转录本,其中26条的表达具有高组织特异性(TSI>0.85),显示大部分高表达基因都表现出高组织特异性。

The leopard cat(Prionailurus bengalensis)is one of the most widespread Asian carnivores,and it is listed as classⅡnationally key protected wild animals in China.In this study,RNA-Seq was performed on 6 tissues(cerebrum,heart,kidney,liver,lung and skeletal muscle)of leopard cat and 51.4 Gb raw data was obtained.De novo assembly by Trinity software obtained a high-quality assembly including 369246 transcripts with an average length of 1465 bp,and Contig N50 has a length of 2660 bp.Annotation results showed that approximately 42.44%(114517)of the transcripts were aligned in 4 public databases.KEGG pathway prediction revealed that 65895 transcripts were assigned to 386 KEGG pathways.According to the expression quantity of transcripts,the expression profiles of 6 tissues were constructed,and the tissue-specific index(TSI)was calculated.The results showed that 39.65%of the transcripts(0.15≤TSI≤0.85)had medium tissue specificity,and 60.34%of the transcripts(TSI>0.85)had high tissue specificity.A total of 39 transcripts were obtained from the top 10 transcripts with the highest FPKM value in each tissue,and 26 of them were highly tissue-specific(TSI>0.85).