装载α-生育酚的乳木果油固体脂质纳米颗粒的制备与表征

Preparation and Evaluation of Shea Butter Solid Lipid Nanoparticles Loaded withα-Tocopherol

以吐温20和吐温80为乳化剂,天然的固体脂质乳木果油为脂质基质,通过高速剪切均质制备乳木果油固体脂质纳米颗粒(solid lipid nanoparticle,SLN)。乳木果油SLN冷却固化温度为4℃;当油水比例为5∶5~1∶9时,动态光散射仪测定乳木果油SLN粒径范围为30.43~278.13 nm,透射电镜观察发现,乳木果油SLN形态为球形。差示扫描量热仪测定结果显示,乳木果油SLN热峰值温度范围为19.05~28.96℃,低于乳木果油的热峰值温度(35.84℃)。而且,以乳木果油SLN为载体包埋亲脂性活性物质α-生育酚,包埋率高达95.11%,因此,将乳木果油SLN用作脂溶性活性物质的载体具有较大的应用潜力。

In this study,we fabricated shea butter solid lipid nanoparticles(SLNs)by a high-speed shear homogenization method with Tween 20 or Tween 80 as the emulsifier.SLNs were obtained by cooling down to 4℃.The particle size range of SLNs with oil to water ratio of 5:5 to 1:9,as determined by dynamic light scattering,was 30.43–278.13 nm.The transmission electron microscopic(TEM)images showed that the particle morphology was spherical.The thermal peak temperature of SLNs ranged from 19.05 to 28.96℃as measured by a differential scanning calorimeter,which was lower than that of shea butter(35.84℃).In addition,α-tocopherol,a lipophilic bioactive ingredient,was loaded into SLNs with encapsulation efficiency as high as 95.11%.Therefore,SLNs has great potential for application as a carrier for liposoluble bioactive materials.