2018—2019年赤道几内亚Bioko岛恶性疟原虫抗药性基因多态性分析

Drug-resistant gene polymorphisms in Plasmodium falciparum isolated from Bioko Island,Equatorial Guinea in 2018 and 2019

目的调查赤道几内亚Bioko岛恶性疟原虫多药耐药蛋白1(Plasmodium falciparum multidrug resistance protein1,PfMDR1)、氯喹抗性转运蛋白基因(P.falciparum chloroquine resistant transporter,PfCRT)和Kelch 13基因(P.falciparum Kelch 13,PfK13)多态性,为当地疟疾防控策略制定提供参考。方法采集2018—2019年赤道几内亚Bioko岛恶性疟原虫感染者外周血样本85份,提取基因组DNA。采用巢式PCR技术扩增、PfCRT和基因,对扩增产物进行DNA测序,并对基因序列进行比对。结果赤道几内亚Bioko岛恶性疟原虫基因不存在已知与青蒿素抗性相关的突变;PfMDR1基因和PfCRT基因均存在不同比例抗药性突变,其中PfMDR1N86Y、PfMDR1Y184F和PfCRTK76T突变率分别为35.29%(30/85)、72.94%(62/85)和24.71%(21/85)。结论赤道几内亚Bioko岛恶性疟原虫PfMDR1、PfCRT基因和基因均存在不同程度突变。

Objective To investigate the genetic polymorphisms of Plasmodium falciparum multidrug resistance protein 1(PfMDR1),chloroquine resistance transporter(PfCRT)and Kelch 13(PfK13)genes in Bioko Island,Equatorial Guinea,so as to provide insights into the development of the malaria control strategy in local areas.Methods A total of 85 peripheral blood samples were collected from patients with Plasmodium falciparum infections in Bioko Island,Equatorial Guinea in 2018 and 2019,and genomic DNA was extracted.The PfMDRl,PfCRT and PfK13 genes were amplified using a nested PCR assay.The amplification products were sequenced,and the gene sequences were aligned.Results There were no mutations associated with artemisinin resistance in PfK13 gene in Bioko Island,Equatorial Guinea,while drug-resistant mutations were detected in PfMDRl and PfCRT genes,and the proportions of PfMDRlN86 Y,PfMDRlY184 F and PfCRTK76 T mutations were 35.29%(30/85),72.94%(62/85)and 24.71%(21/85),respectively.Conclusion There are mutations in PfMDR1,PfCRT and PfK13 genes in P.falciparum isolates from Bioko Island,Equatorial Guinea.