肺腺癌预后不良的重要基因CDC20、CCNB2和CDC45

Important genes for poor prognosis of lung adenocarcinoma:CDC20,CCNB2,and CDC45

目的鉴定与肺腺癌(LUAD)预后相关的基因及潜在机制。方法从基因表达汇编(GEO)数据库中选取基因芯片GSE33532、GSE32863和GSE43458。通过GEO2R和Venn diagram筛选差异表达基因。使用注释、可视化和集成发现在线数据库(DAVID)进行基因本体(GO)分析和京都基因与基因组百科全书(KEGG)分析。通过STRING和Cytoscape构建蛋白-蛋白相互作用网络(PPI),使用MCODE软件进行模块分析。使用Kaplan-Meier Plotter在线数据库进行预后分析。使用基因表达谱互动分析网站(GEPIA)验证基因在LUAD和正常肺组织之间的表达差异。结果共获得331个共同差异表达基因(68个下调基因和263个上调基因)。鉴定出13个核心基因与LUAD预后显著相关并在LUAD中高表达,其中3个基因CDC45、CCNB2和CDC20显著富集在细胞周期途径中,并且显著影响不同临床和病理特征肺癌患者的预后。结论CDC45、CCNB2和CDC20与LUAD预后不良有关,可能是LUAD潜在的治疗靶基因。

Objective To identify genes and potential mechanisms related to the prognosis of lung adenocarcinoma(LUAD).Methods Gene chips GSE33532,GSE32863,and GSE43458 were selected from the Gene Expression Omnibus database.GEO2 R and Venn diagram were used to screen differentially expressed genes.The Database for Annotation,Visualization,and Integrated Discovery(DAVID)was used to perform Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses.A protein-protein interaction(PPI)network was constructed by STRING and Cytoscape,and MCODE was used for module analysis.Kaplan-Meier Plotter was used for prognostic analysis.Gene Expression Profiling Interactive Analysis(GEPIA)was used to validate the differential expression of genes between LUAD and normal lung tissues.Results A total of 331 common differentially expressed genes were obtained,including 68 down-regulated genes and 263 up-regulated genes.Thirteen genes were significantly correlated with the prognosis of LUAD and were highly expressed in LUAD.Among them,3 genes(CDC45,CCNB2,and CDC20)are significantly enriched in the cell cycle pathway,and significantly affect the prognosis of different clinical and pathological factors of lung cancer patients.Conclusion CDC45,CCNB2,and CDC20 are related to poor prognosis of LUAD and are potential therapeutic target genes for lung adenocarcinoma.