摘要
To search naturally occurring interleukin-1β(IL-1β) inhibitors,biscaesalmins A(1) and B(2),two highly oxidized dimeric cassane diterpenoids with a newly formed alicyclic skeleton,have been isolated from the traditional Chinese medicine Kushilian(Caesalpinia minax).Their full structures were determined by comprehensive spectroscopic analysis and quantum chemical TD-DFT(time-dependent density functional theory) calculation.Biosynthetically,1 and 2 were formed via an intermolecular [4+2]Diels-Alder cycloaddition of two monomers,affording an additional six-membered carbon ring linkage.Compounds 1 and 2 inhibited nitric oxide production on lipopolysaccharide-stimulated THP-1 macrophages,with IC_(50) values being at 1.20±0.23 and 2.30±0.15 μmol/L,respectively.Furthermore,compound 1 inhibited NLRP3(NOD-,LRR-and pyrin domain-containing protein 3) inflammasomemediated IL-1β production and blocked the migration of macrophages towards adipocyte conditioned medium.Biscaesalmins A and B might be candidates for treating inflammation-related metabolic diseases.
基金
the financial support of the Science and Technology Development Fund,Macao SAR (No.FDCT 0031/2019/ A1)
the National Natural Science Foundation of China (Nos. 81872754 and 81872756)
University of Macao,Macao SAR (Nos.MYRG2017-00109-ICMS and MYRG2018-00037-ICMS)。
