外周血IL-6 rs1800796基因多态性与胎膜早破并绒毛膜羊膜炎关联性

Correlation between IL-6 rs1800796 gene polymorphism in peripheral blood and premature rupture of membranes complicated with chorioamnionitis

目的探讨外周血白介素-6(Interleukin-6,IL-6)rs1800796基因多态性与胎膜早破(Premature rupture of membranes,PROM)并绒毛膜羊膜炎(Chorioamnionitis,CA)易感性的关系。方法选择2017年9月-2020年9月海南西部中心医院妇产科收治的70例胎膜早破合并绒毛膜羊膜炎产妇为CA组,选择同期医院进行分娩的PROM产妇50例和正常分娩产妇50例分别作为非CA组和对照组,抽取外周静脉血,采用聚合酶链式扩增反应(Polymerase chain amplification reaction,PCR)检测IL-6基因在DNA上的表达,通过测序确定分型结果。常规分离血清后,检测血清中C反应蛋白(C reactive protein,CRP)、降钙素原(Procalcitonin,PCT)和IL-6水平。结果CA组、非CA组产妇GG基因型频率、G等位基因频率(60.00%、70.71%)高于对照组,CC基因型、C等位基因频率(18.57%、29.29%)低于对照组(P<0.05);CA组CC基因型频率、C等位基因频率低于非CA组(P<0.05);CA组携带GG型产妇血清IL-6、CRP、PCT及产后2 h出血量高于GC/CC型(P<0.05);在显性模型、纯合模型和加性模型下,CA发病风险均升高(P<0.05)。结论IL-6基因rs1800796位点基因型GG增加本地区产妇CA易感性,但无法预测PROM和CA发生时间,该结论有待进一步研究。

OBJECTIVE To explore the relationship between interleukin-6(IL-6)rs1800796 gene polymorphism in peripheral blood and premature rupture of membranes(PROM)and chorioamnionitis(CA)susceptibility.METHODS A total of 70 premature rupture of membranes with chorioamnionitis in the Department of Obstetrics and Gynecology of Hainan Western Central Hospital from Sep 2017 to Sep 2020 were enrolled in the CA group,and 50 cases of PROM parturients and 50 cases of normal parturients who gave birth in hospitals during the same period were in the control group.Peripheral venous blood drawn from all the patients was used to detect the DNA expression of IL-6 gene by polymerase chain amplification reaction(PCR),and genotype was confirmed by sequencing.After serum being routinely separated,the levels of C reactive protein(CRP),procalcitonin(PCT)and IL-6 in serum were detected.RESULTS The GG genotype frequency and G allele frequency(60.00%,70.71%)in the CA group and non-CA group were significantly higher than those in the control group,and the CC genotype and C allele frequency(18.57%,29.29%)were significantly lower than those in the control group(P<0.05).The CC genotype frequency and C allele frequency in the CA group were significantly lower than those in the non-CA group(P<0.05).Patients in the CA group carring GG genotype had high levels of maternal serum IL-6,CRP,PCT and postpartum blood loss 2 hours after delivery than those in the patients with GC/CC genotype(P<0.05).Under the prediction of dominant model,homozygous model and additive model,the risk of CA was all significantly increased(P<0.05).CONCLUSION The IL-6 gene rs1800796 locus genotype GG may significantly increase the susceptibility of maternal CA in this area,but it could not predict the occurrence time of PROM and CA,and this conclusion needs further to be studied.