白花丹素调控LINC01615对喉鳞癌细胞生物学行为的影响

Effects of Plumbagin on the Biological Behavior of Laryngeal Squamous Cell Carcinoma Cells by Regulating LINC01615

目的:探讨白花丹素是否通过调控长基因间非编码RNA 01615(LINC01615)进而影响喉鳞癌细胞的增殖、迁移和侵袭。方法:采用细胞计数试剂盒(CCK-8)法、集落形成实验、Transwell实验检测不同剂量(2,4,8μmol·L^(-1))白花丹素对喉鳞癌细胞FD-LSC-1存活、克隆形成以及迁移侵袭的影响;实时定量PCR(RT-qPCR)检测LINC01615表达。将FD-LSC-1细胞分为si-NC组、si-LINC01615组、高剂量(8μmol·L^(-1))药物+pcDNA组、高剂量(8μmol·L^(-1))药物+pcDNA-LINC01615组,采用上述方法检测细胞存活率、克隆形成以及迁移侵袭能力变化。结果:白花丹素中、高剂量白花丹素处理后FD-LSC-1细胞存活率、克隆形成数、迁移和侵袭细胞数、LINC01615及N-cadherin蛋白表达均显著降低,E-cadherin蛋白表达显著升高(P<0.05)。与si-NC组比较,si-LINC01615组FD-LSC-1细胞存活率、克隆形成数、迁移和侵袭细胞数、LINC01615及N-cadherin蛋白表达显著降低,E-cadherin蛋白表达显著升高(P<0.05)。与高剂量药物+pcDNA组比较,高剂量药物+pcDNA-LINC01615组FD-LSC-1细胞存活率、克隆形成数、迁移和侵袭细胞数、LINC01615及N-cadherin蛋白表达显著升高,E-cadherin蛋白表达显著降低(P<0.05)。结论:一定剂量的白花丹素通过下调LINC01615能够抑制喉鳞癌细胞增殖、迁移和侵袭。

Objective:To explore the effects of plumbagin on the proliferation,migration and invasion of laryngeal squamous cell carcinoma cells by regulating long intergenic non-coding RNA 01615(LINC01615).Methods:The cell counting kit(CCK-8)method,colony formation experiment and Transwell experiment were used to detect the effects of plumbagin at different doses(2,4 and 8μmol·L^(-1))on the viability,clone formation,migration and invasion of laryngeal squamous cell carcinoma cells FD-LSC-1,and the expression of LINC01615 was detected by real-time quantitative PCR(RT-qPCR).The FD-LSC-1 cells were divided into si-NC group,si-LINC01615 group,high-dose(8μmol·L^(-1))drug+pcDNA group,and high-dose(8μmol·L^(-1))drug+pcDNA-LINC01615 group,and the above-mentioned methods were used to detect the changes in cell viability,clone formation,and migration and invasion capabilities.Results:The cell viability,clone formation numbers,migration and invasion cell numbers,LINC01615 expression and N-cadherin protein expression of FD-LSC-1 cells were significantly reduced after treated with plumbagin medium at high dose,and E-cadherin protein expression was significantly increased(P<0.05).Compared with those in si-NC group,the cell viability,clone formation numbers,migration and invasion cell numbers,LINC01615 expression and N-cadherin protein expression of FD-LSC-1 cells in si-LINC01615 group were significantly reduced,and E-cadherin protein expression was significantly increased(P<0.05).Compared with those in high-dose drug+pcDNA group,the cell viability,clone formation numbers,migration and invasion cell numbers,LINC01615 expression and N-cadherin expression of FD-LSC-1 cells in high-dose drug+pcDNA-LINC01615 group were significantly increased,and E-cadherin protein expression was significantly reduced(P<0.05).Conclusion:A certain dose of plumbagin can inhibit the proliferation,migration and invasion of laryngeal squamous cell carcinoma cells by down-regulating LINC01615.