产地海拔等对前胡中白花前胡甲、乙含量的影响

Effect of Altitude of Producing Area on Contents of Praeruptorin A and Praeruptorin B in Qianhu(Peucedani Radix)

目的针对目前前胡含量较难合格的问题,对不同产地前胡的质量进行比较分析。方法采用HPLC-DAD法测定前胡中白花前胡甲素、白花前胡乙素的含量,色谱柱为Agilent Eclipse XDB-C18(4.6 mm×250 mm, 5μm),以甲醇-水(75∶25)为流动相,等度洗脱;流速为1.0 mL·min^(-1),柱温25℃,检测波长321 nm,洗脱时间为30 min。并针对实验结果进行了聚类分析、主成分分析。结果 24批不同产地的前胡,白花前胡甲素含量范围在0.83%-1.52%;白花前胡乙素含量范围在0.10%-0.44%,两者均符合规定的占比为45.45%。24批不同产地的前胡聚类分析结果显示,根据海拔可将其分为3类;主成分分析结果显示,综合评分较高的是四川、安徽、重庆、湖北、贵州等高海拔地区。结论由于各地栽培前胡海拔、气候等差异,不同产地高海拔地区前胡的白花前胡甲素和白花前胡乙素含量普遍高于低海拔地区,高海拔地区前胡合格率更高。

Objective To analyze the quality of Qianhu(Peucedani Radix) in different producing areas in view of the difficulty in qualifying the content of Qianhu(Peucedani Radix). Methods HPLC-DAD method was used to determine the contents of Praeruptorin A and Praeruptorin B in Qianhu(Peucedani Radix). Agilent Eclipse XDB-C18(4.6 mm×250 mm, 5 μm) was used as the column and methanol-water(75∶25) was used as the flow phase, with isocratic elution. The flow rate was 1.0 mL·min^(-1). The column temperature was 25 ℃ and the detection wavelength was 321 nm. The elution time was 30 min. According to the experimental results, cluster analysis and principal component analysis were carried out. Results Among the 24 batches of Qianhu(Peucedani Radix) from different producing areas, the contents of Praeruptorin A was in the range of 0.83% to 1.52%, and the content of Praeruptorin B was in the range of 0.10% to 0.44%. The results of cluster analysis showed that they could be divided into 3 categories according to the altitude. The results of principal component analysis showed that the higher comprehensive scores were in high-altitude regions in Sichuan, Anhui, Chongqing, Hubei, Guizhou and other provinces. Conclusion Due to the differences in altitude and climate of the cultivated Qianhu(Peucedani Radix) in different places, the contents of Praeruptorin A and Praeruptorin B in the high-altitude regions of different production areas were generally higher than that in the low-altitude regions, and the pass rate of Qianhu(Peucedani Radix) in the high-altitude regions was higher.