HPLC-MS/MS法测定育发类化妆品中22种禁用物质

Determination of 22 prohibited substances in hair growth cosmetics by HPLC-MS/MS method

建立了高效液相色谱-串联质谱(HPLC-MS/MS)同时测定育发类化妆品中22种禁用物质的分析方法。样品经饱和氯化钠分散后,采用乙腈超声提取,梯度洗脱,多反应监测扫描方式测定,基质匹配标准曲线进行定量分析。结果显示,22种禁用物质在相应的质量浓度范围内成良好的线性关系,相关系数(r)均在0.995以上,检出限(LODs)为4.9~49.7 ng/g,两种基质中的平均回收率为80.7%~110.3%,相对标准偏差(RSD,n=6)均小于15%。本方法简便、灵敏、准确,可用于育发类化妆品中非法添加22种禁用物质的定性及定量测定。

A method of high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was developed for simultaneous determination of 22 prohibited substances in hair growth cosmetics.After dispersed in saturated sodium chloride solution,the samples were ultrasonically extracted with acetonitrile,and separated by gradient elution.The analysis was performed under multiple reaction monitoring(MRM).Matrix-matched standard curve was used for quantitative analysis.The results show that the 22 prohibited substances show good linear relationship within the range of corresponding mass concentrations with the correlation coefficients(r)more than 0.995.The limits of detection(LODs)are 4.9-49.7 ng/g.The average recoveries are within the range of 80.7%-110.3%with the RSD(n=6)less than 15%in the two matrices.With the advantages of simplicity,sensitivity and accuracy,the established method is suitable for the analysis of the 22 prohibited substances in hair growth cosmetics.